3-oxosteroid 1-dehydrogenase:
androstenedione + an oxidized electron carrier→androsta-1,4-diene-3,17-dione + a reduced two electron carrier (EC 1.3.99.4) |
| RR42_RS32000 |
3-oxo-5-alpha-steroid 4-dehydrogenase (in reverse):
androsta-1,4-diene-3,17-dione + a reduced two electron carrier→androsta-1-ene-3,17-dione + an oxidized electron carrier (EC 1.3.99.5) |
| No genes |
| androsta-1-ene-3,17-dione + H2O→1-hydroxyandrostan-3,17-dione (EC 1.17.99.11) |
| No genes |
| 1-hydroxyandrostan-3,17-dione + an oxidized electron carrier→androstan-1,3,17-trione + a reduced two electron carrier (EC 1.17.99.11) |
| No genes |
| androstan-1,3,17-trione + H2O→1,17-dioxo-2,3-seco-androstan-3-oate + H+ |
| No genes |
| 1,17-dioxo-2,3-seco-androstan-3-oate + ATP + coenzyme A→1,17-dioxo-2,3-seco-androstan-3-oyl-CoA + AMP + diphosphate |
| No genes |
| 1,17-dioxo-2,3-seco-androstan-3-oyl-CoA + an oxidized electron carrier→1,17-dioxo-2,3-seco-androsta-4-en-3-oyl-CoA + a reduced two electron carrier |
| No genes |
| 1,17-dioxo-2,3-seco-androsta-4-en-3-oyl-CoA + H2O→4-hydroxy-1,17-dioxo-2,3-seco-androstan-3-oyl-CoA |
| No genes |
| 4-hydroxy-1,17-dioxo-2,3-seco-androstan-3-oyl-CoA→2,5-seco-3,4-dinorandrost-1,5,17-trione + acetyl-CoA |
| No genes |
| 2,5-seco-3,4-dinorandrost-1,5,17-trione→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate |
| No genes |
| 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + ATP + coenzyme A→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphate (EC 6.2.1.41) |
| No genes |
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (in reverse):
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADPH + H+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADP+ (EC 1.1.1.145) |
| RR42_RS31900 |
| 3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + an oxidized electron carrier→(2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + a reduced two electron carrier |
| No genes |
| (2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + H2O→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA + NAD+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 |
| RR42_RS03005 |
| RR42_RS03440 |
| RR42_RS05760 |
| RR42_RS10265 |
| RR42_RS11095 |
| RR42_RS22630 |
| RR42_RS25375 |
| RR42_RS28325 |
| RR42_RS29465 |
| RR42_RS31050 |
| RR42_RS35270 |
| 3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + H2O→3aα-H-4α-carboxy-5-hydroxy-7aβ-methylhexahydro-1-indanone + acetyl-CoA + H+ |
| No genes |
| 3aα-H-4α-carboxy-5-hydroxy-7aβ-methylhexahydro-1-indanone + ATP + coenzyme A→(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA + AMP + diphosphate |
| RR42_RS31940 |
| (3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31940 |
| (5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31900 |
| (5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + H2O→(3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H+ |
| RR42_RS31935 |
| (3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H2O→9-carboxy-6-methyl-3,7-dioxononanoyl-CoA |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
9-carboxy-6-methyl-3,7-dioxononanoyl-CoA + coenzyme A→7-carboxy-4-methyl-5-oxoheptanoyl-CoA + acetyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| 7-carboxy-4-methyl-5-oxoheptanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + a reduced electron-transfer flavoprotein |
| No genes |
| (2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + H2O→(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA (EC 4.2.1.119) |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA + NAD+→7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 |
| RR42_RS03005 |
| RR42_RS03440 |
| RR42_RS05760 |
| RR42_RS10265 |
| RR42_RS11095 |
| RR42_RS22630 |
| RR42_RS25375 |
| RR42_RS28325 |
| RR42_RS29465 |
| RR42_RS31050 |
| RR42_RS35270 |
Acetyl-CoA C-acyltransferase (in reverse):
7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + coenzyme A→5-carboxy-2-methyl-3-oxopentanoyl-CoA + acetyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| 5-carboxy-2-methyl-3-oxopentanoyl-CoA + coenzyme A→succinyl-CoA + propanoyl-CoA |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information