3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
cholesterol + NAD+→cholest-5-en-3-one + NADH + H+ (EC 1.1.1.145) |
| No genes |
Steroid Delta-isomerase:
cholest-5-en-3-one→cholest-4-en-3-one (EC 5.3.3.1) |
| RR42_RS31815 |
| cholest-4-en-3-one + an oxidized electron carrier + H2O→25-hydroxycholest-4-en-3-one + a reduced two electron carrier (EC 1.17.99.10) |
| No genes |
| 25-hydroxycholest-4-en-3-one→(25R)-26-hydroxycholest-4-en-3-one |
| No genes |
| (25R)-26-hydroxycholest-4-en-3-one + an oxidized electron carrier→(25R)-26-oxocholest-4-en-3-one + a reduced two electron carrier |
| No genes |
| (25R)-26-oxocholest-4-en-3-one + NAD+ + H2O→(25R)-3-oxocholest-4-en-26-oate + NADH + 2 H+ |
| No genes |
| (25R)-3-oxocholest-4-en-26-oate[out]→(25R)-3-oxocholest-4-en-26-oate[in] |
| No genes |
| (25R)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25R)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
Alpha-methylacyl-CoA racemase (in reverse):
(25R)-3-oxocholest-4-en-26-oyl-CoA→(25S)-3-oxocholest-4-en-26-oyl-CoA (EC 5.1.99.4) |
| RR42_RS21750 |
| RR42_RS23145 |
| RR42_RS24825 |
| RR42_RS25050 |
| (25S)-3-oxocholest-4-en-26-oyl-CoA + an oxidized electron carrier→3-oxocholest-4,24-dien-26-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxocholest-4,24-dien-26-oyl-CoA + H2O→24-hydroxy-3-oxocholest-4-en-26-oyl-CoA |
| No genes |
| 24-hydroxy-3-oxocholest-4-en-26-oyl-CoA→22-oxocholest-4-en-3-one + propanoyl-CoA |
| No genes |
| 22-oxocholest-4-en-3-one + NAD+ + H2O→3-oxochol-4-en-24-oate + NADH + 2 H+ |
| No genes |
| 3-oxochol-4-en-24-oate + ATP + coenzyme A→3-oxochol-4-en-24-oyl-CoA + AMP + diphosphate |
| No genes |
| 3-oxochol-4-en-24-oyl-CoA + an oxidized electron carrier→3-oxochol-4,22-dien-24-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxochol-4,22-dien-24-oyl-CoA + H2O→22-hydroxy-3-oxochol-4-en-24-oyl-CoA |
| No genes |
| 22-hydroxy-3-oxochol-4-en-24-oyl-CoA→3,23-dioxo-23,24-bisnorchol-4-ene + acetyl-CoA |
| No genes |
| 3,23-dioxo-23,24-bisnorchol-4-ene + NAD+ + H2O→3-oxo-23,24-bisnorchol-4-en-22-oate + NADH + 2 H+ |
| No genes |
| 3-oxo-23,24-bisnorchol-4-en-22-oate + ATP + coenzyme A→3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + AMP + diphosphate |
| No genes |
| 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + an oxidized electron carrier→3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + H2O→17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA |
| No genes |
| 17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA→androstenedione + propanoyl-CoA |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information