Serine--tRNA ligase:
L-serine + ATP + H+→(L-seryl)adenylate + diphosphate (EC 6.1.1.11; 6.1.1.3; 6.1.1.7; 6.2.1.72; 6.3.2.14) |
| RR42_RS04360 |
| RR42_RS06600 |
| RR42_RS15150 |
Cysteine--tRNA ligase:
L-cysteine + ATP + H+→(L-cysteinyl)adenylate + diphosphate (EC 6.1.1.16; 6.2.1.69) |
| RR42_RS06350 |
Proline--tRNA ligase:
L-alanine + ATP + H+→(L-alanyl)adenylate + diphosphate (EC 6.1.1.15; 6.1.1.7; 6.2.1.67) |
| RR42_RS15150 |
| RR42_RS17925 |
Asparagine--tRNA ligase:
L-asparagine + ATP + H+→(L-asparaginyl)adenylate + diphosphate (EC 6.1.1.22) |
| No genes |
| S-adenosyl-L-methionine + ATP + H+→(S-adenosyl-L-methionyl)adenylate + diphosphate (EC 6.2.1.M38) |
| No genes |
| (L-cysteinyl)adenylate + a holo [ClbK polyketide synthase]→L-cysteinyl-[ClbK polyketide synthase] + AMP + H+ (EC 6.2.1.69) |
| No genes |
| [ClbG aminomalonyltransferase]-aminomalonyl-L-serine + a holo [ClbO polyketide synthase]→aminomalonyl-[ClbO polyketide synthase] + a [ClbG aminomalonyltransferase]-L-serine |
| No genes |
Alanine--tRNA ligase:
glycine + ATP→(glycyl)adenylate + diphosphate (EC 6.1.1.7; 6.2.1.66) |
| RR42_RS15150 |
| (L-asparaginyl)adenylate + a holo [ClbN polyketide synthase]→L-asparaginyl-[ClbN polyketide synthase] + AMP + H+ |
| No genes |
| (L-alanyl)adenylate + a holo [ClbB polyketide synthase]→an L-alanyl-[ClbB polyketide synthase] + AMP + H+ (EC 6.2.1.67) |
| No genes |
| (S-adenosyl-L-methionyl)adenylate + a holo [ClbH non-ribosomal peptide synthase]→S-adenosyl-L-methionyl-[ClbH non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.M38) |
| No genes |
| (glycyl)adenylate + a holo [ClbJ non-ribosomal peptide synthase]→glycyl-[ClbJ non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.66) |
| No genes |
| (L-cysteinyl)adenylate + a holo [ClbJ non-ribosomal peptide synthase]→L-cysteinyl-[ClbJ non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.69) |
| No genes |
| (L-seryl)adenylate + a holo-[L-seryl-carrier protein ClbE]→L-seryl-[L-seryl-carrier protein ClbE] + AMP + H+ (EC 6.2.1.72) |
| No genes |
| L-asparaginyl-[ClbN polyketide synthase] + myristoyl-CoA→myristoyl-D-asparaginyl-[ClbN polyketide synthase] + coenzyme A + H+ |
| No genes |
| L-seryl-[L-seryl-carrier protein ClbE] + NAD+→L-3-oxoalanyl-[L-seryl-carrier protein ClbE] + NADH + H+ |
| No genes |
| an L-alanyl-[ClbB polyketide synthase] + myristoyl-D-asparaginyl-[ClbN polyketide synthase]→myristoyl-D-asparaginyl-L-alanyl-[ClbB polyketide synthase] + a holo [ClbN polyketide synthase] + H+ |
| No genes |
| L-3-oxoalanyl-[L-seryl-carrier protein ClbE] + FAD + H2O→aminomalonyl-[L-seryl-carrier protein ClbE] + FADH2 |
| No genes |
| myristoyl-D-asparaginyl-L-alanyl-[ClbB polyketide synthase] + malonyl-CoA + 2 NADPH + 3 H+→myristoyl-D-asparaginyl-butan-2-aminyl-acetyl-[ClbB polyketide synthase] + CO2 + coenzyme A + 2 NADP+ + H2O |
| No genes |
| aminomalonyl-[L-seryl-carrier protein ClbE] + a [ClbG aminomalonyltransferase]-L-serine→[ClbG aminomalonyltransferase]-aminomalonyl-L-serine + a holo-[L-seryl-carrier protein ClbE] |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-acetyl-[ClbB polyketide synthase] + a holo [ClbC polyketide synthase] + malonyl-CoA + H+→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-[ClbC polyketide synthase] + CO2 + coenzyme A + a holo [ClbB polyketide synthase] |
| No genes |
| [ClbG aminomalonyltransferase]-aminomalonyl-L-serine + a holo [ClbK polyketide synthase]→aminomalonyl-[ClbK polyketide synthase] + a [ClbG aminomalonyltransferase]-L-serine |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-[ClbC polyketide synthase] + S-adenosyl-L-methionyl-[ClbH non-ribosomal peptide synthase]→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-SAM-[ClbH non-ribosomal peptide synthase] + a holo [ClbB polyketide synthase] + H+ |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-SAM-[ClbH non-ribosomal peptide synthase]→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-[ClbH non-ribosomal peptide synthase] + S-methyl-5'-thioadenosine + H+ |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-[ClbH non-ribosomal peptide synthase] + a holo [ClbI polyketide synthase] + malonyl-CoA + H+→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-[ClbI polyketide synthase] + CO2 + coenzyme A + a holo [ClbH non-ribosomal peptide synthase] |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-[ClbI polyketide synthase] + glycyl-[ClbJ non-ribosomal peptide synthase]→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-glycyl-[ClbJ non-ribosomal peptide synthase] + a holo [ClbI polyketide synthase] |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-glycyl-[ClbJ non-ribosomal peptide synthase] + L-cysteinyl-[ClbJ non-ribosomal peptide synthase]→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-4,5-dihydro-1,3-thiazole-4-carboxyl-[ClbJ non-ribosomal peptide synthase] + a holo [ClbJ non-ribosomal peptide synthase] + H+ + H2O |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-glycyl-[ClbJ non-ribosomal peptide synthase] + H2O→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-glycine + a holo [ClbJ non-ribosomal peptide synthase] + H+ |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-glycine + H2O→glycine + myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetate |
| No genes |
| aminomalonyl-[ClbK polyketide synthase] + myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-4,5-dihydro-1,3-thiazole-4-carboxyl-[ClbJ non-ribosomal peptide synthase] + NADP+→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-glycyl-[ClbK polyketide synthase] + CO2 + a holo [ClbJ non-ribosomal peptide synthase] + NADPH + H+ |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-glycyl-[ClbK polyketide synthase] + L-cysteinyl-[ClbK polyketide synthase] + NADP+→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-2-(aminomethyl)-1,3-thiazole-4-carboxyl-[ClbK polyketide synthase] + a holo [ClbK polyketide synthase] + NADPH + 2 H+ + H2O |
| No genes |
| aminomalonyl-[ClbO polyketide synthase] + myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-2-(aminomethyl)-1,3-thiazole-4-carboxyl-[ClbK polyketide synthase]→myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-2-(aminomethyl)-1,3-thiazole-4-carboxyl-glycyl-[ClbO polyketide synthase] + CO2 + a holo [ClbK polyketide synthase] |
| No genes |
| myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetyl-aminomethyl-1,3-thiazole-4-carboxy-2-(aminomethyl)-1,3-thiazole-4-carboxyl-glycyl-[ClbO polyketide synthase] + myristoyl-D-asparaginyl-butan-2-aminyl-malonyl-1-aminocyclopropane-1-carboxyl-acetate + 2 H+→a holo [ClbO polyketide synthase] + precolibactin 1491 + CO2 |
| No genes |
| precolibactin 1491[in] + Na+[out]→precolibactin 1491[out] + Na+[in] |
| No genes |
| precolibactin 1491 + 2 H2O→unstable precolibactin intermediate + 2 N-myristoyl-D-asparagine |
| No genes |
| unstable precolibactin intermediate→precolibactin 16a + 2 H+ + 4 H2O (spontaneous) |
| No genes |
| precolibactin 16a + an oxidized electron carrier→precolibactin 16b + a reduced two electron carrier + H+ |
| No genes |
| precolibactin 16b + H+ + H2O→colibactin + ammonium (spontaneous) |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information