MetaCyc Pathway: (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Proline--tRNA ligase:
L-alanine + ATP + H+→(L-alanyl)adenylate + diphosphate (EC 6.1.1.15; 6.1.1.7; 6.2.1.67)
RR42_RS15150
RR42_RS17925
Glutamate--tRNA ligase:
L-glutamate + ATP + H+→(L-glutamyl)adenylate + diphosphate (EC 6.1.1.17; 6.1.1.24; 6.2.1.68)
RR42_RS13355
(L-alanyl)adenylate + an AmbB non-ribosomal peptide synthase→L-alanyl-[AmbB non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.67)
No genes
(L-glutamyl)adenylate + an ambE non-ribosomal peptide synthase→L-glutamyl-[ambE non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.68)
No genes
L-alanyl-[AmbB non-ribosomal peptide synthase] + L-glutamyl-[ambE non-ribosomal peptide synthase]→L-alanyl-L-glutamyl-[AmbE non-ribosomal peptide synthase] + an AmbB non-ribosomal peptide synthase + H+
No genes
L-alanyl-[AmbB non-ribosomal peptide synthase] + an ambE non-ribosomal peptide synthase→L-alanyl-[AmbE non-ribosomal peptide synthase] + an AmbB non-ribosomal peptide synthase
No genes
L-alanyl-[AmbE non-ribosomal peptide synthase] + L-alanyl-L-glutamyl-[AmbE non-ribosomal peptide synthase]→L-alanyl-L-glutamyl-L-alanyl--[AmbE non-ribosomal peptide synthase] + an ambE non-ribosomal peptide synthase + H+
No genes
L-alanyl-L-glutamyl-L-alanyl--[AmbE non-ribosomal peptide synthase]→L-alanyl-(2S,3E)-amino-4-methoxy-but-3-enoyl-L-alanyl-[AmbE non-ribosomal peptide synthase]
No genes
L-alanyl-(2S,3E)-amino-4-methoxy-but-3-enoyl-L-alanyl-[AmbE non-ribosomal peptide synthase] + H2O→L-alanyl-(2S,3E)-amino-4-methoxy-but-3-enoyl-L-alanine + an ambE non-ribosomal peptide synthase + H+
No genes
L-alanyl-(2S,3E)-amino-4-methoxy-but-3-enoyl-L-alanine + 2 H2O→2 L-alanine + (2S,3E)-2-amino-4-methoxy-but-3-enoate
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information