MetaCyc Pathway: tRNA-uridine 2-thiolation (thermophilic bacteria) in Cupriavidus basilensis FW507-4G11

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a TtuB sulfur-carrier protein + ATP + H+→a carboxyadenylated [TtuB sulfur-carrier protein] + diphosphate (EC 2.7.7.M15)
No genes
Cysteine desulfurase:
L-cysteine + an [L-cysteine desulfurase]-L-cysteine→an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
RR42_RS06025
RR42_RS21580
RR42_RS26680
RR42_RS26805
RR42_RS34005
an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + a [TtuD protein]-L-cysteine→a [TtuD protein]-S-sulfanyl-L-cysteine + an [L-cysteine desulfurase]-L-cysteine (EC 2.8.1.M4)
No genes
a carboxyadenylated [TtuB sulfur-carrier protein] + a [TtuD protein]-S-sulfanyl-L-cysteine + a reduced two electron carrier→a thiocarboxylated [TtuB sulfur-carrier protein] + AMP + an oxidized electron carrier + H+ + a [TtuD protein]-L-cysteine (EC 2.8.1.M3)
No genes
a thiocarboxylated [TtuB sulfur-carrier protein] + ATP + H2O + a 5-methyluracil54 in tRNA→a TtuB sulfur-carrier protein + a 5-methyl-2-thiouracil54 in tRNA + AMP + diphosphate + 2 H+ (EC 2.8.1.15)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information