MetaCyc Pathway: holomycin biosynthesis in Cupriavidus basilensis FW507-4G11

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L-cysteine + ATP + an HlmE non-ribosomal peptide synthetase→an L-cysteinyl-[HlmE non-ribosomal peptide synthetase] + AMP + diphosphate
No genes
an L-cysteinyl-[HlmE non-ribosomal peptide synthetase] + an oxidized electron carrier→a (2E)-2-amino-3-sulfanylprop-2-enoyl-[HlmE non-ribosomal peptide synthetase] + a reduced two electron carrier
RR42_RS16795
a (2E)-2-amino-3-sulfanylprop-2-enoyl-[HlmE non-ribosomal peptide synthetase] + L-cysteine + ATP + an oxidized electron carrier→a (2E)-2-[(2E)-2-azanidyl-3-sulfanylprop-2-enamido]-3-[HlmE non-ribosomal peptide synthetase] + AMP + a reduced two electron carrier + diphosphate + H+
No genes
a (2E)-2-[(2E)-2-azanidyl-3-sulfanylprop-2-enamido]-3-[HlmE non-ribosomal peptide synthetase][cytosol]→4-amino-2-(methanethioyl)-5-oxo-3-sulfanylpyrrolidine-2-carboxyl-[HlmE non-ribosomal peptide synthetase][cytosol]
No genes
4-amino-2-(methanethioyl)-5-oxo-3-sulfanylpyrrolidine-2-carboxyl-[HlmE non-ribosomal peptide synthetase] + H2O→4-amino-2-(methanethioyl)-5-oxo-3-sulfanylpyrrolidine-2-carboxylate + an HlmE non-ribosomal peptide synthetase + H+
No genes
4-amino-2-(methanethioyl)-5-oxo-3-sulfanylpyrrolidine-2-carboxylate + H+→(5E)-3-amino-4-sulfanyl-5-(sulfanylmethylidene)pyrrolidin-2-one + CO2
No genes
(5E)-3-amino-4-sulfanyl-5-(sulfanylmethylidene)pyrrolidin-2-one + an oxidized electron carrier→(5E)-3-imino-4-sulfanyl-5-(sulfanylmethylidene)pyrrolidin-2-one + a reduced two electron carrier + H+
No genes
(5E)-3-imino-4-sulfanyl-5-(sulfanylmethylidene)pyrrolidin-2-one→dithioholothin (spontaneous)
No genes
dithioholothin + dioxygen→holothin + hydrogen peroxide (EC 1.8.3.M1)
No genes
dithioholothin + acetyl-CoA→dithioholomycin + coenzyme A
No genes
holothin + acetyl-CoA→holomycin + coenzyme A
No genes
dithioholomycin + dioxygen→holomycin + hydrogen peroxide (EC 1.8.3.M1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information