MetaCyc Pathway: coumarins biosynthesis (engineered) in Cupriavidus basilensis FW507-4G11

Add experiment(s):


L-tyrosine + FADH2 + dioxygen→L-dopa + FAD + H+ + H2O (EC 1.14.14.9)
No genes
Tyrosine ammonia-lyase:
L-tyrosine→4-coumarate + ammonium (EC 4.3.1.23; 4.3.1.25; 5.4.3.6)
RR42_RS01710
4-coumarate--CoA ligase:
4-coumarate + ATP + coenzyme A→(E)-4-coumaroyl-CoA + AMP + diphosphate (EC 6.2.1.12)
RR42_RS36410
Tyrosine ammonia-lyase:
L-dopa→trans-caffeate + ammonium (EC 4.3.1.23; 4.3.1.25)
No genes
4-coumarate + FADH2 + dioxygen→trans-caffeate + FAD + H+ + H2O (EC 1.14.14.9)
No genes
4-coumarate--CoA ligase:
trans-caffeate + ATP + coenzyme A→(E)-caffeoyl-CoA + AMP + diphosphate (EC 6.2.1.12)
RR42_RS36410
(E)-4-coumaroyl-CoA + 2-oxoglutarate + dioxygen→2,4-dihydroxycinnamoyl-CoA + CO2 + succinate (EC 1.14.11.62)
No genes
Caffeoyl-CoA O-methyltransferase:
(E)-caffeoyl-CoA + S-adenosyl-L-methionine→feruloyl-CoA + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.104)
No genes
2,4-dihydroxycinnamoyl-CoA→umbelliferone + coenzyme A (spontaneous)
No genes
(E)-caffeoyl-CoA + 2-oxoglutarate + dioxygen→2,4,6-trihydroxycinnamoyl-CoA + CO2 + succinate
No genes
feruloyl-CoA + 2-oxoglutarate + dioxygen→trans-6-hydroxyferuloyl-CoA + CO2 + succinate (EC 1.14.11.61)
No genes
2,4,6-trihydroxycinnamoyl-CoA→esculetin + coenzyme A
No genes
trans-6-hydroxyferuloyl-CoA→scopoletin + coenzyme A (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information