MetaCyc Pathway: superpathway of steroid hormone biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Cholesterol monooxygenase (side-chain-cleaving):
cholesterol + dioxygen + 2 H+ + 2 a reduced adrenodoxin→(22R)-22-hydroxycholesterol + 2 an oxidized adrenodoxin + H2O (EC 1.14.15.6)
No genes
Cholesterol monooxygenase (side-chain-cleaving):
(22R)-22-hydroxycholesterol + dioxygen + 2 H+ + 2 a reduced adrenodoxin→(20R,22R)-dihydroxycholesterol + 2 an oxidized adrenodoxin + H2O (EC 1.14.15.6)
No genes
Cholesterol monooxygenase (side-chain-cleaving):
(20R,22R)-dihydroxycholesterol + dioxygen + 2 H+ + 2 a reduced adrenodoxin→pregnenolone + 4-methylpentanal + 2 an oxidized adrenodoxin + 2 H2O (EC 1.14.15.6)
No genes
pregnenolone[in]→pregnenolone[out]
No genes
pregnenolone + dioxygen + a reduced [NADPH-hemoprotein reductase]→17-α-hydroxypregnenolone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.19)
No genes
3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
pregnenolone + NAD+→pregn-5-ene-3,20-dione + NADH + H+ (EC 1.1.1.145)
No genes
17-α-hydroxypregnenolone + dioxygen + a reduced [NADPH-hemoprotein reductase]→3-β-hydroxyandrost-5-en-17-one + acetate + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.32)
No genes
Steroid Delta-isomerase:
pregn-5-ene-3,20-dione→progesterone (EC 5.3.3.1)
RR42_RS31815
3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
3-β-hydroxyandrost-5-en-17-one + NAD+→androst-5-ene-3,17-dione + NADH + H+ (EC 1.1.1.145)
No genes
progesterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→17-α-hydroxyprogesterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.19)
No genes
progesterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→11-deoxycorticosterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.16)
No genes
17-α-hydroxyprogesterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→11-desoxycortisol + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.16)
No genes
Steroid Delta-isomerase:
androst-5-ene-3,17-dione→androstenedione (EC 5.3.3.1)
RR42_RS31815
11-deoxycorticosterone[in]→11-deoxycorticosterone[out]
No genes
Testosterone 17-beta-dehydrogenase (NADP(+)) (in reverse):
androstenedione + NADPH + H+→testosterone + NADP+ (EC 1.1.1.64)
No genes
androstenedione + dioxygen + a reduced [NADPH-hemoprotein reductase]→19-hydroxyandrostenedione + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.14)
No genes
Steroid 11-beta-monooxygenase:
11-deoxycorticosterone + dioxygen + 2 H+ + 2 a reduced adrenodoxin→corticosterone + 2 an oxidized adrenodoxin + H2O (EC 1.14.15.4)
No genes
11-desoxycortisol[in]→11-desoxycortisol[out]
No genes
Cholestenone 5-alpha-reductase (in reverse):
testosterone + NADPH + H+→5α-dihydrotestosterone + NADP+ (EC 1.3.1.22)
No genes
19-hydroxyandrostenedione + dioxygen + a reduced [NADPH-hemoprotein reductase]→19-oxoandrostenedione + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.14.14)
No genes
testosterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→19-hydroxytestosterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.14)
No genes
Steroid 11-beta-monooxygenase:
11-desoxycortisol + dioxygen + 2 H+ + 2 a reduced adrenodoxin→cortisol + 2 an oxidized adrenodoxin + H2O (EC 1.14.15.4)
No genes
Corticosterone 18-monooxygenase:
corticosterone + dioxygen + 2 H+ + 2 a reduced adrenodoxin→18-hydroxycorticosterone + 2 an oxidized adrenodoxin + H2O (EC 1.14.15.5)
No genes
19-oxoandrostenedione + dioxygen + a reduced [NADPH-hemoprotein reductase]→estrone + formate + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.14)
No genes
19-hydroxytestosterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→19-oxo-testosterone + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.14.14)
No genes
Corticosterone 18-monooxygenase:
18-hydroxycorticosterone + dioxygen + 2 H+ + 2 a reduced adrenodoxin→aldosterone + 2 an oxidized adrenodoxin + 2 H2O (EC 1.14.15.5)
No genes
Estradiol 17-beta-dehydrogenase (in reverse):
estrone + NADPH + H+→17β-estradiol + NADP+ (EC 1.1.1.62)
No genes
19-oxo-testosterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→17β-estradiol + formate + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information