MetaCyc Pathway: L-ascorbate biosynthesis VIII (engineered pathway) in Cupriavidus basilensis FW507-4G11

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Quinoprotein glucose dehydrogenase:
D-glucopyranose[out] + a ubiquinone[pm-bac-neg]→D-glucono-1,5-lactone[out] + a ubiquinol[pm-bac-neg] (EC 1.1.5.2)
No genes
Gluconolactonase:
D-glucono-1,5-lactone + H2O→D-gluconate + H+ (spontaneous) (EC 3.1.1.17)
RR42_RS09940
RR42_RS23435
RR42_RS26550
RR42_RS27020
RR42_RS33665
Gluconate 2-dehydrogenase (acceptor):
D-gluconate + an oxidized electron carrier→2-keto-D-gluconate + a reduced two electron carrier (EC 1.1.99.3)
RR42_RS10775
2,5-didehydrogluconate reductase:
2-keto-D-gluconate + NADP+→2,5-didehydro-D-gluconate + NADPH + H+ (EC 1.1.1.274)
RR42_RS35195
Dehydrogluconate dehydrogenase:
2-keto-D-gluconate + an oxidized electron carrier→2,5-didehydro-D-gluconate + a reduced two electron carrier (EC 1.1.99.4)
No genes
2,5-didehydro-D-gluconate + NADPH + H+→2-keto-L-gulonate + NADP+ (EC 1.1.1.346)
RR42_RS35195
2-keto-L-gulonate→L-ascorbate + H2O
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information