1-deoxy-D-xylulose-5-phosphate synthase:
D-glyceraldehyde 3-phosphate + pyruvate + H+→1-deoxy-D-xylulose 5-phosphate + CO2 (EC 2.2.1.7) |
| RR42_RS15005 |
1-deoxy-D-xylulose-5-phosphate reductoisomerase (in reverse):
1-deoxy-D-xylulose 5-phosphate + NADPH + H+→2-C-methyl-D-erythritol 4-phosphate + NADP+ (EC 1.1.1.267) |
| RR42_RS11845 |
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase:
2-C-methyl-D-erythritol 4-phosphate + CTP + H+→4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + diphosphate (EC 2.7.7.60) |
| RR42_RS07850 |
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase:
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP→2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP + H+ (EC 2.7.1.148) |
| RR42_RS02025 |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase:
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol→2-C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP (EC 4.6.1.12) |
| RR42_RS07855 |
| 2-C-methyl-D-erythritol-2,4-cyclodiphosphate + a reduced flavodoxin→(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + an oxidized flavodoxin + H+ + H2O (EC 1.17.7.3) |
| No genes |
| (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + a reduced flavodoxin→prenyl diphosphate + an oxidized flavodoxin + H2O (EC 1.17.7.4) |
| RR42_RS16985 |
| RR42_RS32230 |
| (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + a reduced flavodoxin→3-methylbut-3-en-1-yl diphosphate + an oxidized flavodoxin + H2O (EC 1.17.7.4) |
| RR42_RS16985 |
| RR42_RS32230 |
Isopentenyl-diphosphate Delta-isomerase:
3-methylbut-3-en-1-yl diphosphate→prenyl diphosphate (EC 5.3.3.2) |
| No genes |
Dimethylallyltranstransferase:
prenyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranyl diphosphate + diphosphate (EC 2.5.1.1) |
| RR42_RS15010 |
Geranyltranstransferase:
geranyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→(2E,6E)-farnesyl diphosphate + diphosphate (EC 2.5.1.10) |
| RR42_RS15010 |
| 2 (2E,6E)-farnesyl diphosphate→presqualene diphosphate + diphosphate (EC 2.5.1.103; 2.5.1.21; 2.5.1.96) |
| RR42_RS07905 |
| RR42_RS26935 |
| RR42_RS32265 |
Squalene synthase:
presqualene diphosphate + NAD(P)H + H+→squalene + NAD(P)+ + diphosphate (EC 2.5.1.21) |
| RR42_RS07905 |
| RR42_RS26935 |
| RR42_RS32265 |
| squalene + dioxygen + a reduced [NADPH-hemoprotein reductase]→(3S)-2,3-epoxy-2,3-dihydrosqualene + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.17) |
| No genes |
Cycloartenol synthase:
(3S)-2,3-epoxy-2,3-dihydrosqualene→cycloartenol (EC 5.4.99.8) |
| No genes |
Cycloartenol 24-C-methyltransferase:
cycloartenol + S-adenosyl-L-methionine→cyclolaudenol + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.142) |
| No genes |
| cyclolaudenol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4β-carboxycyclolaudenol + 6 a ferricytochrome b5 + 4 H2O |
| No genes |
| 4β-carboxycyclolaudenol + H+→4α, 14α-dimethyl ergosta-8,25(27)-dienol + CO2 |
| No genes |
| 4α, 14α-dimethyl ergosta-8,25(27)-dienol + 3 dioxygen + 3 a reduced [NADPH-hemoprotein reductase]→4α-methyl ergosta-8,14,25(27)-trienol + formate + 3 an oxidized [NADPH-hemoprotein reductase] + H+ + 4 H2O (EC 1.14.14.154) |
| No genes |
Delta(14)-sterol reductase (in reverse):
4α-methyl ergosta-8,14,25(27)-trienol + NADPH + H+→4α-methyl ergosta-8,25(27)-dienol + NADP+ (EC 1.3.1.70) |
| No genes |
| 4α-methyl ergosta-8,25(27)-dienol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-ergosta-8,25(27)-dienol + 6 a ferricytochrome b5 + 4 H2O |
| No genes |
| 4α-carboxy-ergosta-8,25(27)-dienol + H+→ergosta-8,25(27)-dienol + CO2 |
| No genes |
| ergosta-8,25(27)-dienol→ergosta-7,25(27)-dienol |
| No genes |
| ergosta-7,25(27)-dienol + NADPH + H+→fungisterol + NADP+ |
| No genes |
| fungisterol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→ergosta-5,7-dienol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20) |
| No genes |
| ergosta-5,7-dienol + NADP+→ergosterol + NADPH + H+ |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information