MetaCyc Pathway: nicotine degradation III (VPP pathway) in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Nicotine dehydrogenase:
(S)-nicotine + an oxidized pseudoazurin + H2O→(S)-6-hydroxynicotine + 2 H+ + a reduced pseudoazurin (EC 1.5.99.4)
RR42_RS02360
(S)-6-hydroxynicotine oxidase:
(S)-6-hydroxynicotine + dioxygen→6-hydroxy-N-methylmyosmine + hydrogen peroxide (EC 1.5.3.5)
No genes
(S)-6-hydroxynicotine oxidase:
6-hydroxy-N-methylmyosmine + H2O→6-hydroxypseudooxynicotine (spontaneous) (EC 1.5.3.5; 1.5.3.6)
No genes
6-hydroxypseudooxynicotine + an oxidized electron-transfer flavoprotein + H+ + H2O→4-(6-hydroxy-pyridin-3-yl)-4-oxobutanal + a reduced electron-transfer flavoprotein + methylamine (EC 1.4.98.M1)
No genes
4-(6-hydroxy-pyridin-3-yl)-4-oxobutanal + NADP+ + H2O→4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + NADPH + 2 H+
No genes
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + dioxygen + 2 H+→2,5-dihydroxypyridine + 2 NAD+ + succinate semialdehyde + H2O (EC 1.14.13.163)
No genes
2,5-dihydroxypyridine 5,6-dioxygenase:
2,5-dihydroxypyridine + dioxygen→N-formylmaleamate + H+ (EC 1.13.11.9)
RR42_RS21640
RR42_RS26485
N-formylmaleamate + H2O→maleamate + formate + H+ (EC 3.5.1.106)
RR42_RS21635
RR42_RS26480
maleamate + H2O→maleate + ammonium (EC 3.5.1.107)
RR42_RS21645
RR42_RS26490
Maleate isomerase:
maleate→fumarate (EC 5.2.1.1)
RR42_RS21630
RR42_RS26475

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information