MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
RR42_RS02175
RR42_RS05435
RR42_RS05440
RR42_RS13670
RR42_RS26245
RR42_RS29140
RR42_RS31370
RR42_RS36655
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
RR42_RS05445
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
RR42_RS16210
RR42_RS18585
RR42_RS31070
RR42_RS33305
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
RR42_RS09745
RR42_RS10210
RR42_RS10545
RR42_RS11005
RR42_RS18350
RR42_RS24070
RR42_RS24560
RR42_RS25355
RR42_RS25775
RR42_RS28650
RR42_RS32920
RR42_RS34260
RR42_RS34620
RR42_RS36940

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information