MetaCyc Pathway: nicotine degradation II (pyrrolidine pathway) in Cupriavidus basilensis FW507-4G11

Add experiment(s):


(S)-nicotine + 2 an oxidized c-type cytochrome→N-methylmyosmine + 2 a reduced c-type cytochrome + 2 H+ (EC 1.4.2.2)
No genes
N-methylmyosmine + H2O→pseudooxynicotine (spontaneous)
No genes
pseudooxynicotine + 2 an oxidized c-type cytochrome + H2O→4-oxo-4-(pyridin-3-yl)butanal + 2 a reduced c-type cytochrome + methylamine + 2 H+ (EC 1.4.2.3)
No genes
4-oxo-4-(pyridin-3-yl)butanal + NADP+ + H2O→4-oxo-4-(3-pyridyl)-butanoate + NADPH + 2 H+ (EC 1.2.1.83)
No genes
4-oxo-4-(3-pyridyl)-butanoate + an oxidized electron carrier + H2O→4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + a reduced two electron carrier
RR42_RS26495
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + dioxygen + 2 H+→2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O (EC 1.14.13.163)
No genes
2,5-dihydroxypyridine 5,6-dioxygenase:
2,5-dihydroxypyridine + dioxygen→N-formylmaleamate + H+ (EC 1.13.11.9)
RR42_RS21640
RR42_RS26485
succinate semialdehyde + NADP+ + H2O→succinate + NADPH + 2 H+ (EC 1.2.1.79)
No genes
N-formylmaleamate + H2O→maleamate + formate + H+ (EC 3.5.1.106)
RR42_RS21635
RR42_RS26480
maleamate + H2O→maleate + ammonium (EC 3.5.1.107)
RR42_RS21645
RR42_RS26490
Maleate isomerase:
maleate→fumarate (EC 5.2.1.1)
RR42_RS21630
RR42_RS26475

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information