3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
cholesterol + NAD+→cholest-5-en-3-one + NADH + H+ (EC 1.1.1.145) |
| No genes |
Steroid Delta-isomerase:
cholest-5-en-3-one→cholest-4-en-3-one (EC 5.3.3.1) |
| RR42_RS31815 |
| cholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-hydroxycholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| cholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-26-hydroxycholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.28) |
| No genes |
| (25S)-26-hydroxycholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-oxocholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.29) |
| No genes |
| (25R)-26-hydroxycholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-26-oxocholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.28) |
| No genes |
| (25S)-26-oxocholest-4-en-3-one + dioxygen + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-3-oxocholest-4-en-26-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| (25R)-26-oxocholest-4-en-3-one + dioxygen + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-3-oxocholest-4-en-26-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.28) |
| No genes |
| (25S)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25S)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
| (25R)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25R)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
Alpha-methylacyl-CoA racemase (in reverse):
(25R)-3-oxocholest-4-en-26-oyl-CoA→(25S)-3-oxocholest-4-en-26-oyl-CoA (EC 5.1.99.4) |
| RR42_RS21750 |
| RR42_RS23145 |
| RR42_RS24825 |
| RR42_RS25050 |
| (25S)-3-oxocholest-4-en-26-oyl-CoA + an oxidized electron carrier→3-oxocholest-4,24-dien-26-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxocholest-4,24-dien-26-oyl-CoA + H2O→24-hydroxy-3-oxocholest-4-en-26-oyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
24-hydroxy-3-oxocholest-4-en-26-oyl-CoA + NAD+→3,24-dioxocholest-4-en-26-oyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 |
| RR42_RS03005 |
| RR42_RS03440 |
| RR42_RS05760 |
| RR42_RS10265 |
| RR42_RS11095 |
| RR42_RS22630 |
| RR42_RS25375 |
| RR42_RS28325 |
| RR42_RS29465 |
| RR42_RS31050 |
| RR42_RS35270 |
Propanoyl-CoA C-acyltransferase (in reverse):
3,24-dioxocholest-4-en-26-oyl-CoA + coenzyme A→3-oxochol-4-en-24-oyl-CoA + propanoyl-CoA (EC 2.3.1.176) |
| No genes |
| 3-oxochol-4-en-24-oyl-CoA + an oxidized electron carrier→3-oxochol-4,22-dien-24-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxochol-4,22-dien-24-oyl-CoA + H2O→22-hydroxy-3-oxochol-4-en-24-oyl-CoA |
| No genes |
| 22-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+→3,22-dioxochol-4-en-24-oyl-CoA + NADH + H+ |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
3,22-dioxochol-4-en-24-oyl-CoA + coenzyme A→acetyl-CoA + 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + an oxidized electron carrier→3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + H2O→17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA |
| No genes |
| 17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA→propanoyl-CoA + androstenedione |
| No genes |
3-oxosteroid 1-dehydrogenase:
androstenedione + an oxidized electron carrier→androsta-1,4-diene-3,17-dione + a reduced two electron carrier (EC 1.3.99.4) |
| RR42_RS32000 |
| androsta-1,4-diene-3,17-dione + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→9α-hydroxyandrosta-1,4-diene-3,17-dione + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.30) |
| No genes |
| 9α-hydroxyandrosta-1,4-diene-3,17-dione→3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione (spontaneous) |
| No genes |
| 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + dioxygen→3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H+ + H2O (EC 1.14.14.12) |
| No genes |
dioxygenase:
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + dioxygen→(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1 (10),2-dien-4-oate + H+ (EC 1.13.11.25) |
| RR42_RS31960 |
| (1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1 (10),2-dien-4-oate + H2O→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H+ (EC 3.7.1.17) |
| RR42_RS31825 |
| (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxohexanoate (EC 4.2.1.132) |
| RR42_RS31850 |
| 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + ATP + coenzyme A→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphate (EC 6.2.1.41) |
| No genes |
| (S)-4-hydroxy-2-oxohexanoate→1-propanal + pyruvate (EC 4.1.3.43) |
| RR42_RS31860 |
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (in reverse):
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADPH + H+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADP+ (EC 1.1.1.145) |
| RR42_RS31900 |
| 1-propanal + coenzyme A + NAD+→propanoyl-CoA + NADH + H+ (EC 1.2.1.87) |
| RR42_RS31855 |
| 3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + an oxidized electron carrier→(2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + a reduced two electron carrier |
| No genes |
| (2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + H2O→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA + NAD+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 |
| RR42_RS03005 |
| RR42_RS03440 |
| RR42_RS05760 |
| RR42_RS10265 |
| RR42_RS11095 |
| RR42_RS22630 |
| RR42_RS25375 |
| RR42_RS28325 |
| RR42_RS29465 |
| RR42_RS31050 |
| RR42_RS35270 |
Acetyl-CoA C-acyltransferase (in reverse):
3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + coenzyme A→acetyl-CoA + (3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| (3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31940 |
| (5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31900 |
| (5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + H2O→(3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H+ |
| RR42_RS31935 |
| (3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H2O→9-carboxy-6-methyl-3,7-dioxononanoyl-CoA |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
9-carboxy-6-methyl-3,7-dioxononanoyl-CoA + coenzyme A→acetyl-CoA + 7-carboxy-4-methyl-5-oxoheptanoyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| 7-carboxy-4-methyl-5-oxoheptanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + a reduced electron-transfer flavoprotein |
| No genes |
| (2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + H2O→(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA (EC 4.2.1.119) |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA + NAD+→7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 |
| RR42_RS03005 |
| RR42_RS03440 |
| RR42_RS05760 |
| RR42_RS10265 |
| RR42_RS11095 |
| RR42_RS22630 |
| RR42_RS25375 |
| RR42_RS28325 |
| RR42_RS29465 |
| RR42_RS31050 |
| RR42_RS35270 |
Acetyl-CoA C-acyltransferase (in reverse):
7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + coenzyme A→acetyl-CoA + 5-carboxy-2-methyl-3-oxopentanoyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS28055 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
| 5-carboxy-2-methyl-3-oxopentanoyl-CoA + coenzyme A→propanoyl-CoA + succinyl-CoA |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information