MetaCyc Pathway: methylaspartate cycle in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: methylaspartate cycle in Cupriavidus basilensis FW507-4G11

Aconitate hydratase:
citrate→cis-aconitate + H₂O (EC 4.2.1.3)

Aconitate hydratase:
cis-aconitate + H₂O→D-threo-isocitrate (EC 4.2.1.3)

Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP⁺→2-oxoglutarate + CO₂ + NADPH (EC 1.1.1.42)

Glutamate dehydrogenase (in reverse):
2-oxoglutarate + ammonium + NADH + H⁺→L-glutamate + NAD⁺ + H₂O (EC 1.4.1.2)

Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)

No genes

Methylaspartate ammonia-lyase:
(2S, 3S)-3-methylaspartate→mesaconate + ammonium (EC 4.3.1.2)

No genes

mesaconate + succinyl-CoA→2-methylfumaryl-CoA + succinate (EC 2.8.3.26)

2-methylfumaryl-CoA + H₂O→(2R,3S)-β-methylmalyl-CoA (EC 4.2.1.148)

No genes

Malyl-CoA lyase:
(2R,3S)-β-methylmalyl-CoA→glyoxylate + propanoyl-CoA (EC 4.1.3.24)

Malyl-CoA lyase (in reverse):
glyoxylate + acetyl-CoA→(S)-malyl-CoA (EC 4.1.3.24)

Propionyl-CoA carboxylase:
propanoyl-CoA + ATP + hydrogen carbonate→(S)-methylmalonyl-CoA + ADP + H⁺ + phosphate (EC 6.4.1.3)

Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)

No genes

(S)-malyl-CoA + H₂O→(S)-malate + coenzyme A + H⁺ (EC 3.1.2.30)

No genes

Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)

Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)

Succinate dehydrogenase (ubiquinone):
succinate_[in] + an electron-transfer quinone→fumarate_[in] + an electron-transfer quinol (EC 1.3.5.1)

Fumarate hydratase (in reverse):
fumarate + H₂O→(S)-malate (EC 4.2.1.2)

Malate dehydrogenase:
(S)-malate + NAD⁺→oxaloacetate + NADH + H⁺ (EC 1.1.1.37; 1.1.1.38)

Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H₂O→citrate + coenzyme A + H⁺ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information