MetaCyc Pathway: superpathway of ergotamine biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Proline--tRNA ligase:
L-alanine + ATP + H+→(L-alanyl)adenylate + diphosphate (EC 6.1.1.15; 6.1.1.7; 6.2.1.67)
RR42_RS15150
RR42_RS17925
Proline--tRNA ligase:
L-proline + ATP + H+→(L-prolyl)adenylate + diphosphate (EC 6.1.1.15; 6.2.1.53)
RR42_RS17925
Phenylalanine--tRNA ligase:
L-phenylalanine + ATP + H+→(L-phenylalanyl)adenylate + diphosphate (EC 6.1.1.20)
RR42_RS06620
RR42_RS06625
Tryptophan dimethylallyltransferase:
L-tryptophan + prenyl diphosphate→4-prenyl-L-tryptophan + diphosphate (EC 2.5.1.34)
No genes
4-prenyl-L-tryptophan + S-adenosyl-L-methionine→4-prenyl-L-abrine + S-adenosyl-L-homocysteine + 2 H+ (EC 2.1.1.261)
No genes
4-prenyl-L-abrine + dioxygen + H+→chanoclavin diene intermediate + hydrogen peroxide
No genes
chanoclavin diene intermediate + NADPH + dioxygen + H+→chanoclavin epoxide intermediate + NADP+ + H2O
No genes
chanoclavin epoxide intermediate + H+→chanoclavine-I + CO2 (spontaneous)
No genes
chanoclavine-I + NAD+→chanoclavine-I aldehyde + NADH + H+ (EC 1.1.1.332)
No genes
chanoclavine-I aldehyde→6,8-dimethyl-6,7,8,9-tetradehydroergoline + H2O (spontaneous)
No genes
6,8-dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+→agroclavine + NADP+ (EC 1.5.1.46)
No genes
agroclavine + NADPH + dioxygen + H+→elymoclavine + NADP+ + H2O
No genes
elymoclavine + dioxygen + a reduced [NADPH-hemoprotein reductase]→elymoclavine aldehyde + an oxidized [NADPH-hemoprotein reductase] + 2 H2O
No genes
elymoclavine aldehyde + dioxygen + a reduced [NADPH-hemoprotein reductase]→paspalate + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O
No genes
paspalate→lysergic acid
No genes
lysergic acid + ATP + H+→lysergic acid adenylate + diphosphate
No genes
(L-alanyl)adenylate + (L-prolyl)adenylate + (L-phenylalanyl)adenylate + lysergic acid adenylate→ergotamam + 4 AMP + 7 H+
No genes
ergotamam + 2-oxoglutarate + dioxygen→ergotamine + CO2 + succinate
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information