MetaCyc Pathway: phosphinothricin tripeptide biosynthesis in Cupriavidus basilensis FW507-4G11

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Phosphoenolpyruvate mutase:
phosphoenolpyruvate + H+→3-phosphonopyruvate (EC 5.4.2.9)
RR42_RS22445
Proline--tRNA ligase:
L-alanine + ATP + H+→(L-alanyl)adenylate + diphosphate (EC 6.1.1.15; 6.1.1.7; 6.2.1.67)
RR42_RS15150
RR42_RS17925
Phosphonopyruvate decarboxylase:
3-phosphonopyruvate + H+→phosphonoacetaldehyde + CO2 (EC 4.1.1.82)
RR42_RS22450
(L-alanyl)adenylate + a PhsB non-ribosomal peptide synthase→L-alanyl-[PhsB non-ribosomal peptide synthase] + AMP + H+ (EC 6.2.1.67)
No genes
phosphonoacetaldehyde + NADH + H+→2-hydroxyethylphosphonate + NAD+ (EC 1.1.1.309)
No genes
2-hydroxyethylphosphonate + dioxygen→(hydroxymethyl)phosphonate + formate + H+ (EC 1.13.11.72)
No genes
(hydroxymethyl)phosphonate + NADP+→phosphonoformaldehyde + NADPH + 2 H+
No genes
phosphonoformaldehyde + NADP+ + H2O→phosphonoformate + NADPH + H+
No genes
phosphonoformate + CTP→CMP-5'-phosphonoformate + diphosphate (EC 2.7.7.93)
No genes
CMP-5'-phosphonoformate + 3-phospho-D-glycerate + H2O→2-phosphono-formylglycerate + CMP + H+ + phosphate
No genes
2-phosphono-formylglycerate→1-carboxyvinyl carboxyphosphonate + H2O
No genes
Carboxyvinyl-carboxyphosphonate phosphorylmutase:
1-carboxyvinyl carboxyphosphonate→carboxyphosphinopyruvate (EC 2.7.8.23)
No genes
Carboxyvinyl-carboxyphosphonate phosphorylmutase:
carboxyphosphinopyruvate + H+→3-(hydroxyphosphinoyl)pyruvate + CO2 (EC 2.7.8.23)
No genes
3-(hydroxyphosphinoyl)pyruvate + acetyl-CoA + H2O→2-(phosphonoylmethyl)malate + coenzyme A + H+ (EC 2.3.3.18)
No genes
2-(phosphonoylmethyl)malate→3-(hydroxyphosphonoylmethyl)malate (EC 4.2.1.166)
No genes
3-(hydroxyphosphonoylmethyl)malate + an oxidized electron carrier + H+→deamino α-keto demethylphosphinothricin + CO2 + a reduced two electron carrier
No genes
deamino α-keto demethylphosphinothricin + an aminated amino group donor→demethyl-L-phosphinothricin + a deaminated amino group donor
No genes
Phosphinothricin acetyltransferase:
demethyl-L-phosphinothricin + acetyl-CoA→N-acetyldemethyl-L-phosphinothricin + coenzyme A + H+ (EC 2.3.1.183)
RR42_RS10890
RR42_RS19775
N-acetyldemethyl-L-phosphinothricin + ATP + H+→N-acetyldemethylphosphinothricin adenylate + diphosphate
No genes
N-acetyldemethylphosphinothricin adenylate + a PhsA non-ribosomal peptide synthase→N-acetyldemethylphosphinothricinyl-[PhsA non-ribosomal peptide synthase] + AMP + H+
No genes
N-acetyldemethylphosphinothricinyl-[PhsA non-ribosomal peptide synthase] + L-alanyl-[PhsB non-ribosomal peptide synthase]→N-acetyldemethylphosphinothricinyl-L-alanyl-[PhsB non-ribosomal peptide synthase] + H+ + a PhsA non-ribosomal peptide synthase
No genes
N-acetyldemethylphosphinothricinyl-L-alanyl-[PhsB non-ribosomal peptide synthase] + L-alanine + ATP + a PhsC non-ribosomal peptide synthase→N-acetyldemethylphosphinothricinyl-L-alanyl-L-alanyl-[PhsC non-ribosomal peptide synthase] + AMP + diphosphate + H+ + a PhsB non-ribosomal peptide synthase
No genes
N-acetyldemethylphosphinothricinyl-L-alanyl-L-alanyl-[PhsC non-ribosomal peptide synthase] + H2O→N-acetyldemethylphosphinothricin tripeptide + H+ + a PhsC non-ribosomal peptide synthase
No genes
N-acetyldemethylphosphinothricin tripeptide + a reduced two electron carrier + 2 S-adenosyl-L-methionine→N-acetylphosphinothricin tripeptide + S-adenosyl-L-homocysteine + an oxidized electron carrier + 5'-deoxyadenosine + L-methionine + 2 H+ (EC 2.1.1.326)
No genes
N-acetylphosphinothricin tripeptide + H2O→bialaphos + acetate
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information