MetaCyc Pathway: superpathway of unsaturated fatty acids biosynthesis (<i>E. coli</i>) in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: superpathway of unsaturated fatty acids biosynthesis (E. coli) in Cupriavidus basilensis FW507-4G11

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)

No genes

Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H⁺→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H⁺→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H⁺→a (5Z)-dodec-5-enoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-dodec-5-enoyl-[acp] + a malonyl-[acp] + H⁺→a (7Z)-3-oxotetradec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxotetradec-7-enoyl-[acp] + NADPH + H⁺→a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp]→a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + NADH + H⁺→a (7Z)-tetradec-7-enoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-tetradec-7-enoyl-[acp] + a malonyl-[acp] + H⁺→a (9Z)-3-oxohexadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxohexadec-9-enoyl-[acp] + NADPH + H⁺→a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp]→a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + NADH + H⁺→a palmitoleoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a palmitoleoyl-[acp] + a malonyl-[acp] + H⁺→an (11Z)-3-oxooctadec-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoleoyl-[acp] + H₂O→palmitoleate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (11Z)-3-oxooctadec-11-enoyl-[acp] + NADPH + H⁺→a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp]→a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + NADH + H⁺→a cis-vaccenoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Oleoyl-[acyl-carrier-protein] hydrolase:
a cis-vaccenoyl-[acp] + H₂O→cis-vaccenate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information