MetaCyc Pathway: hypoglycin biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O
(EC 4.3.1.19)
RR42_RS02315
RR42_RS05695
RR42_RS11110
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate
(spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
RR42_RS02315
RR42_RS05695
RR42_RS11110
RR42_RS32990
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
RR42_RS02315
RR42_RS05695
RR42_RS11110
RR42_RS32990
RR42_RS34135
2-oxobutanoate→5-hydroxy-2-oxopentanoate
No genes
5-hydroxy-2-oxopentanoate→2-oxopent-4-enoate + H2O
No genes
1.-.-.-:
2-oxopent-4-enoate + an oxidized electron carrier→2-oxopenta-3,4-dienoate + a reduced two electron carrier
No genes
2-oxopenta-3,4-dienoate→2-oxohexa-4,5-cyclopropyl-5-enoate
No genes
2-oxohexa-4,5-cyclopropyl-5-enoate + acetyl-CoA + H2O→3-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate + coenzyme A + H+
No genes
2-oxohexa-4,5-cyclopropyl-5-enoate + an aminated amino group donor→L-α-(methylenecyclopropyl)-glycine + a deaminated amino group donor
No genes
3-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate→2-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate
No genes
2-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate + an oxidized electron carrier→2-oxo-3-carboxy-4,5-cyclopropylhex-5-enoate + a reduced two electron carrier
No genes
2-oxo-3-carboxy-4,5-cyclopropylhex-5-enoate + H+→β-methylenecyclopropyl pyruvate + CO2
No genes
β-methylenecyclopropyl pyruvate + L-alanine→hypoglycin A + pyruvate
No genes
Gamma-glutamyltransferase:
hypoglycin A + glutathione→hypoglycin B + L-cysteinylglycine
(EC 2.3.2.2)
RR42_RS05040
RR42_RS15195
RR42_RS23025
RR42_RS26175
RR42_RS27305
RR42_RS32275

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information