MetaCyc Pathway: (5R)-carbapenem carboxylate biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


L-proline[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.2)
RR42_RS20125
(S)-1-pyrroline-5-carboxylate + malonyl-CoA + H+ + H2O→(2S,5S)-5-carboxymethyl proline + CO2 + coenzyme A (EC 2.3.1.226)
No genes
(2S,5S)-5-carboxymethyl proline + ATP→(3S,5S)-carbapenam-3-carboxylate + AMP + diphosphate + H+ (EC 6.3.3.6)
No genes
(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + dioxygen→(5R)-carbapen-2-em-3-carboxylate + CO2 + succinate + H2O (EC 1.14.20.3)
No genes
(3S,5S)-carbapenam-3-carboxylate→(3S,5R)-carbapenam
No genes
(3S,5R)-carbapenam + an oxidized electron carrier→(5R)-carbapen-2-em-3-carboxylate + a reduced two electron carrier
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information