Glutamate--tRNA ligase:
L-glutamate + ATP + a tRNAglu→an L-glutamyl-[tRNAGlu] + AMP + diphosphate (EC 6.1.1.17) |
| RR42_RS13355 |
| 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + a reduced two electron carrier + S-adenosyl-L-methionine→5-hydroxybenzimidazole + ammonium + an oxidized electron carrier + 5'-deoxyadenosine + formate + L-methionine + 2 H+ + phosphate (EC 4.1.99.23) |
| No genes |
| L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177) |
| No genes |
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2 (EC 4.1.1.81) |
| RR42_RS16090 |
Glutamyl-tRNA reductase (in reverse):
an L-glutamyl-[tRNAGlu] + NADPH + H+→(S)-4-amino-5-oxopentanoate + a tRNAglu + NADP+ (EC 1.2.1.70) |
| RR42_RS18465 |
| 5-hydroxybenzimidazole + S-adenosyl-L-methionine→5-methoxybenzimidazole + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M9) |
| No genes |
Glutamate-1-semialdehyde 2,1-aminomutase:
(S)-4-amino-5-oxopentanoate→5-aminolevulinate (EC 5.4.3.8) |
| RR42_RS04235 |
| RR42_RS15790 |
| 5-methoxybenzimidazole + S-adenosyl-L-methionine→5-methoxy-6-methylbenzimidazole + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M10) |
| No genes |
Porphobilinogen synthase:
2 5-aminolevulinate→porphobilinogen + H+ + 2 H2O (EC 4.2.1.24) |
| RR42_RS19100 |
| 5-methoxy-6-methylbenzimidazole→5,6-dimethylbenzimidazole |
| No genes |
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase:
5,6-dimethylbenzimidazole + β-nicotinate D-ribonucleotide→α-ribazole 5'-phosphate + nicotinate + H+ (EC 2.4.2.21) |
| RR42_RS16120 |
Hydroxymethylbilane synthase:
4 porphobilinogen + H2O→preuroporphyrinogen + 4 ammonium (EC 2.5.1.61) |
| RR42_RS15900 |
Uroporphyrinogen-III synthase:
preuroporphyrinogen→uroporphyrinogen-III + H2O (EC 4.2.1.75) |
| RR42_RS15895 |
Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.107) |
| RR42_RS15895 |
| RR42_RS16245 |
Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine (EC 2.1.1.107) |
| RR42_RS15895 |
| RR42_RS16245 |
Precorrin-2 dehydrogenase:
precorrin-2 + NAD+→sirohydrochlorin + NADH + 2 H+ (EC 1.3.1.76) |
| RR42_RS16240 |
| RR42_RS16245 |
Sirohydrochlorin cobaltochelatase:
sirohydrochlorin + Co2+→cobalt-sirohydrochlorin + 2 H+ (EC 4.99.1.3) |
| RR42_RS16240 |
Cobalt-factor II C(20)-methyltransferase:
cobalt-sirohydrochlorin + S-adenosyl-L-methionine→cobalt-factor III + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.151) |
| No genes |
| cobalt-factor III + a reduced two electron carrier→cobalt-precorrin-3 + an oxidized electron carrier (EC 2.1.1.272) |
| No genes |
| cobalt-precorrin-3 + S-adenosyl-L-methionine→cobalt-precorrin-4 + S-adenosyl-L-homocysteine (EC 2.1.1.272) |
| No genes |
| cobalt-precorrin-4 + S-adenosyl-L-methionine→cobalt-precorrin-5A + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.271) |
| No genes |
| cobalt-precorrin-5A + H2O→cobalt-precorrin-5B + acetaldehyde + H+ (EC 3.7.1.12) |
| No genes |
| cobalt-precorrin-5B + S-adenosyl-L-methionine→cobalt-precorrin-6A + S-adenosyl-L-homocysteine (EC 2.1.1.195) |
| No genes |
| cobalt-precorrin-6A + NADH→cobalt-precorrin-6B + NAD+ (EC 1.3.1.106) |
| No genes |
| cobalt-precorrin-6B + H+ + S-adenosyl-L-methionine→cobalt-precorrin-7 + S-adenosyl-L-homocysteine + CO2 (EC 2.1.1.196) |
| RR42_RS30545 |
| cobalt-precorrin-7 + S-adenosyl-L-methionine→cobalt-precorrin-8 + S-adenosyl-L-homocysteine (EC 2.1.1.289) |
| No genes |
| cobalt-precorrin-8→cobyrinate + H+ (EC 5.4.99.60) |
| No genes |
| cobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→cob(II)yrinate a,c-diamide + 2 L-glutamate + 2 ADP + 2 H+ + 2 phosphate (EC 6.3.5.11) |
| RR42_RS35070 |
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
cob(II)yrinate a,c-diamide + a [corrinoid adenosyltransferase]→cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase] (EC 2.5.1.17) |
| RR42_RS16125 |
| RR42_RS17280 |
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase] + a [reduced flavoprotein]→2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + an [oxidized flavoprotein] + 2 H+ (spontaneous) (EC 2.5.1.17) |
| RR42_RS16125 |
| RR42_RS17280 |
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + 2 ATP + 2 H+→2 adenosyl-cob(III)yrinate a,c-diamide + 2 a [corrinoid adenosyltransferase] + 2 triphosphate (EC 2.5.1.17) |
| RR42_RS16125 |
| RR42_RS17280 |
Adenosylcobyric acid synthase (glutamine-hydrolyzing):
adenosyl-cob(III)yrinate a,c-diamide + 4 ATP + 4 L-glutamine + 4 H2O→adenosylcobyrate + 4 L-glutamate + 4 ADP + 4 H+ + 4 phosphate (EC 6.3.5.10) |
| RR42_RS16075 |
Adenosylcobinamide-phosphate synthase:
adenosylcobyrate + (R)-1-amino-2-propanol O-2-phosphate + ATP→adenosyl-cobinamide phosphate + ADP + H+ + phosphate (EC 6.3.1.10) |
| RR42_RS14790 |
| RR42_RS16085 |
Adenosylcobinamide-phosphate guanylyltransferase:
adenosyl-cobinamide phosphate + GTP + H+→adenosylcobinamide-GDP + diphosphate (EC 2.7.7.62) |
| RR42_RS16080 |
Adenosylcobinamide-GDP ribazoletransferase:
adenosylcobinamide-GDP + α-ribazole 5'-phosphate→adenosylcobalamin 5'-phosphate + GMP + H+ (EC 2.7.8.26) |
| RR42_RS16115 |
Alpha-ribazole phosphatase:
adenosylcobalamin 5'-phosphate + H2O→adenosylcobalamin + phosphate (EC 3.1.3.73) |
| RR42_RS16110 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information