MetaCyc Pathway: tropane alkaloids biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Aromatic-amino-acid transaminase (in reverse):
L-phenylalanine + 3-(4-hydroxyphenyl)pyruvate→3-phenyl-2-oxopropanoate + L-tyrosine (EC 2.6.1.57)
RR42_RS04465
RR42_RS05990
RR42_RS33490
1-methylpyrrolinium + 2 malonyl-CoA + H+ + H2O→4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate + 2 CO2 + 2 coenzyme A
No genes
Indolelactate dehydrogenase (in reverse):
3-phenyl-2-oxopropanoate + NADH + H+→(R)-3-(phenyl)lactate + NAD+ (EC 1.1.1.110)
No genes
4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate + H+→hygrine + CO2
No genes
4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoate + dioxygen + H+ + a reduced [NADPH-hemoprotein reductase]→tropinone + CO2 + an oxidized [NADPH-hemoprotein reductase] + 2 H2O
No genes
(R)-3-(phenyl)lactate + UDP-α-D-glucose→(R)-3-(phenyl)lactate glucoside + UDP
No genes
Tropinone reductase I (in reverse):
tropinone + NADPH + H+→tropine + NADP+ (EC 1.1.1.206)
No genes
Tropine acyltransferase:
(R)-3-(phenyl)lactate glucoside + tropine→littorine + D-glucopyranose (EC 2.3.1.185)
No genes
littorine→(S)-atropine
No genes
Hyoscyamine (6S)-dioxygenase:
(S)-atropine + 2-oxoglutarate + dioxygen→(6S)-hydroxyhyoscyamine + CO2 + succinate (EC 1.14.11.11)
No genes
(6S)-hydroxyhyoscyamine + 2-oxoglutarate + dioxygen→scopolamine + CO2 + H+ + succinate + H2O (EC 1.14.20.13)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information