| p-cumate + NADH + dioxygen + H+→(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+ (EC 1.14.12.25) |
| No genes |
2,3-dihydroxy-2,3-dihydro-p-cumate dehydrogenase:
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+→2,3-dihydroxy-p-cumate + NADH + H+ (EC 1.3.1.58) |
| No genes |
| 2,3-dihydroxy-p-cumate + dioxygen→2-hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate + H+ (EC 1.13.11.M5) |
| No genes |
| 2-hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate + H+→2-hydroxy-7-methyl-6-oxo-2,4-octadienoate + CO2 (EC 4.1.1.M8) |
| No genes |
| 2-hydroxy-7-methyl-6-oxo-2,4-octadienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + isobutanoate + H+ |
| No genes |
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate (EC 4.2.1.80) |
| RR42_RS05105 |
| RR42_RS27885 |
| RR42_RS32645 |
| RR42_RS34355 |
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate (EC 4.1.3.39) |
| RR42_RS05095 |
| RR42_RS27900 |
| RR42_RS31860 |
| RR42_RS32625 |
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10) |
| RR42_RS05100 |
| RR42_RS27895 |
| RR42_RS31855 |
| RR42_RS32630 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information