MetaCyc Pathway: toluene degradation VI (anaerobic) in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: toluene degradation VI (anaerobic) in Cupriavidus basilensis FW507-4G11

Benzylsuccinate synthase (in reverse):
toluene + fumarate→(R)-2-benzylsuccinate (EC 4.1.99.11)

No genes

Succinyl-CoA:(R)-benzylsuccinate CoA-transferase:
(R)-2-benzylsuccinate + succinyl-CoA→(R)-benzylsuccinyl-CoA + succinate (EC 2.8.3.15)

No genes

(R)-benzylsuccinyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(E)-2-benzylidenesuccinyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.3)

(E)-2-benzylidenesuccinyl-CoA + H₂O→(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA (EC 4.2.1.180)

No genes

(2S)-2-[(R)-α-hydroxybenzyl]succinyl-CoA + NAD⁺→(S)-2-benzoylsuccinyl-CoA + NADH + H⁺ (EC 1.1.1.429)

No genes

(S)-2-benzoylsuccinyl-CoA + coenzyme A→benzoyl-CoA + succinyl-CoA (EC 2.3.1.310)

No genes

benzoyl-CoA + 2 ATP + 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H₂O→cyclohex-1,5-diene-1-carbonyl-CoA + 2 ADP + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 phosphate (EC 1.3.7.8)

No genes

Cyclohexa-1,5-dienecarbonyl-CoA hydratase (in reverse):
cyclohex-1,5-diene-1-carbonyl-CoA + H₂O→6-hydroxycyclohex-1-ene-1-carbonyl-CoA (EC 4.2.1.100)

No genes

6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD⁺→6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H⁺ (EC 1.1.1.368)

No genes

6-oxocyclohex-1-ene-1-carbonyl-CoA + H₂O→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA (EC 3.7.1.21)

No genes

2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H₂O→3-hydroxypimeloyl-CoA + H⁺ (EC 3.7.1.21)

No genes

3-hydroxypimeloyl-CoA dehydrogenase:
3-hydroxypimeloyl-CoA + NAD⁺→3-oxopimeloyl-CoA + NADH + H⁺ (EC 1.1.1.259)

No genes

3-oxopimeloyl-CoA + coenzyme A→glutaryl-CoA + acetyl-CoA

glutaryl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)

No genes

(E)-glutaconyl-CoA + H⁺→crotonyl-CoA + CO₂ (EC 1.3.8.6)

No genes

crotonyl-CoA + H₂O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD⁺→acetoacetyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information