| toluene + NADH + dioxygen + H+→2-methylphenol + NAD+ + H2O (EC 1.14.13.243) |
| RR42_RS32665 |
| toluene + NADH + dioxygen + H+→4-methylphenol + NAD+ + H2O (EC 1.14.13.236) |
| No genes |
Toluene dioxygenase:
toluene + NADH + dioxygen + H+→(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ (EC 1.14.12.11) |
| No genes |
| toluene + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→benzyl alcohol + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.26) |
| No genes |
Aryl-alcohol dehydrogenase:
benzyl alcohol + NAD+→benzaldehyde + NADH + H+ (EC 1.1.1.90) |
| No genes |
| 2-methylphenol + NADH + dioxygen + H+→3-methylcatechol + NAD+ + H2O (EC 1.14.13.243) |
| RR42_RS32665 |
| 4-methylphenol + 2 an oxidized azurin + H2O→4-hydroxybenzyl alcohol + 2 H+ + 2 a reduced azurin (EC 1.17.9.1) |
| No genes |
Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase:
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+→3-methylcatechol + NADH + H+ (EC 1.3.1.19) |
| No genes |
Benzaldehyde dehydrogenase (NAD(+)):
benzaldehyde + NAD+ + H2O→benzoate + NADH + 2 H+ (EC 1.2.1.28) |
| No genes |
Catechol 2,3-dioxygenase:
3-methylcatechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H+ (EC 1.13.11.2) |
| RR42_RS32655 |
| RR42_RS34600 |
| 4-hydroxybenzyl alcohol + 2 an oxidized azurin→4-hydroxybenzaldehyde + 2 H+ + 2 a reduced azurin (EC 1.17.9.1) |
| No genes |
| (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + acetate + H+ (EC 3.7.1.25) |
| No genes |
Benzoate 1,2-dioxygenase:
benzoate + NADH + dioxygen + H+→3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+ (EC 1.14.12.10) |
| RR42_RS23490 |
| RR42_RS23495 |
| RR42_RS23500 |
| 4-hydroxybenzaldehyde + NADP+ + H2O→4-hydroxybenzoate + NADPH + 2 H+ (EC 1.2.1.96) |
| No genes |
1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase:
3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+→catechol + CO2 + NADH (EC 1.3.1.25) |
| RR42_RS23485 |
4-hydroxybenzoate 3-monooxygenase:
4-hydroxybenzoate + NADPH + dioxygen + H+→protocatechuate + NADP+ + H2O (EC 1.14.13.2) |
| RR42_RS21940 |
Catechol 2,3-dioxygenase:
catechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ (EC 1.13.11.2) |
| RR42_RS32655 |
| RR42_RS34600 |
Protocatechuate 3,4-dioxygenase:
protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H+ (EC 1.13.11.3) |
| RR42_RS32060 |
| RR42_RS32065 |
2-hydroxymuconate-semialdehyde hydrolase:
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + formate + H+ (EC 3.7.1.9) |
| RR42_RS25595 |
| RR42_RS34060 |
3-carboxy-cis,cis-muconate cycloisomerase (in reverse):
3-carboxy-cis,cis-muconate + H+→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2) |
| RR42_RS25850 |
| RR42_RS32055 |
| (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + NAD+ + H2O→(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+ (EC 1.2.1.85) |
| No genes |
4-carboxymuconolactone decarboxylase:
(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H+→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO2 (EC 4.1.1.44) |
| RR42_RS02890 |
| RR42_RS23415 |
| RR42_RS32050 |
| RR42_RS35470 |
| (2Z,4E)-2-hydroxyhexa-2,4-dienedioate→(3E)-2-oxohex-3-enedioate (EC 5.3.2.6) |
| RR42_RS11545 |
| RR42_RS32635 |
3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+ (EC 3.1.1.24) |
| RR42_RS00755 |
| RR42_RS10565 |
| RR42_RS10690 |
| RR42_RS21850 |
| RR42_RS24710 |
| RR42_RS30700 |
| RR42_RS32050 |
4-oxalocrotonate decarboxylase:
(3E)-2-oxohex-3-enedioate + H+→(2Z)-2-hydroxypenta-2,4-dienoate + CO2 (EC 4.1.1.77) |
| RR42_RS05105 |
| RR42_RS31850 |
| RR42_RS32640 |
| RR42_RS32645 |
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate (EC 4.2.1.80) |
| RR42_RS05105 |
| RR42_RS27885 |
| RR42_RS32645 |
| RR42_RS34355 |
3-oxoadipate CoA-transferase:
3-oxoadipate + succinyl-CoA→3-oxoadipoyl-CoA + succinate (EC 2.8.3.6) |
| RR42_RS10005 |
| RR42_RS10010 |
| RR42_RS22965 |
| RR42_RS22970 |
| RR42_RS31950 |
| RR42_RS31955 |
| RR42_RS35920 |
| RR42_RS35925 |
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate (EC 4.1.3.39) |
| RR42_RS05095 |
| RR42_RS27900 |
| RR42_RS31860 |
| RR42_RS32625 |
3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→acetyl-CoA + succinyl-CoA (EC 2.3.1.174) |
| RR42_RS35915 |
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10) |
| RR42_RS05100 |
| RR42_RS27895 |
| RR42_RS31855 |
| RR42_RS32630 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information