MetaCyc Pathway: toluene degradation I (aerobic) (via o-cresol) in Cupriavidus basilensis FW507-4G11

toluene + NADH + dioxygen + H+→2-methylphenol + NAD+ + H2O (EC 1.14.13.243)

RR42_RS32665

2-methylphenol + NADH + dioxygen + H+→3-methylcatechol + NAD+ + H2O (EC 1.14.13.243)

RR42_RS32665

Catechol 2,3-dioxygenase: 3-methylcatechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H+ (EC 1.13.11.2)

RR42_RS32655

RR42_RS34600

(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + acetate + H+ (EC 3.7.1.25)

No genes

2-oxopent-4-enoate hydratase (in reverse): (2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate (EC 4.2.1.80)

RR42_RS05105

RR42_RS27885

RR42_RS32645

RR42_RS34355

4-hydroxy-2-oxovalerate aldolase: (S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate (EC 4.1.3.39)

RR42_RS05095

RR42_RS27900

RR42_RS31860

RR42_RS32625

Acetaldehyde dehydrogenase (acetylating): acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)

RR42_RS05100

RR42_RS27895

RR42_RS31855

RR42_RS32630

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information