MetaCyc Pathway: L-isoleucine biosynthesis V in Cupriavidus basilensis FW507-4G11

Acetate--CoA ligase: 2-methylbutanoate + ATP + coenzyme A→(2S)-2-methylbutanoyl-CoA + AMP + diphosphate (EC 6.2.1.1)

RR42_RS06210

RR42_RS10650

RR42_RS13880

RR42_RS22715

RR42_RS24890

RR42_RS29035

3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse): (2S)-2-methylbutanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(3S)-3-methyl-2-oxopentanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)

RR42_RS19540

Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

RR42_RS25890

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information