MetaCyc Pathway: L-isoleucine biosynthesis II in Cupriavidus basilensis FW507-4G11

Add experiment(s):


pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase:
(R)-citramalate→citraconate + H2O (EC 4.2.1.35)
RR42_RS01530
RR42_RS01535
RR42_RS14390
RR42_RS14400
RR42_RS21080
RR42_RS21085
RR42_RS22770
RR42_RS22775
RR42_RS24175
RR42_RS24180
RR42_RS33965
citraconate + H2O→(2R,3S)-3-methylmalate
RR42_RS14400
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
RR42_RS14385
Acetolactate synthase:
2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
RR42_RS02175
RR42_RS05435
RR42_RS05440
RR42_RS13670
RR42_RS26245
RR42_RS29140
RR42_RS31370
RR42_RS36655
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
RR42_RS16210
RR42_RS18585
RR42_RS31070
RR42_RS33305
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
RR42_RS25890

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information