MetaCyc Pathway: 2-oxoisovalerate decarboxylation to isobutanoyl-CoA in Cupriavidus basilensis FW507-4G11

2-oxoisovalerate dehydrogenase (acylating):
a [BCAA dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + 3-methyl-2-oxobutanoate + H⁺→an [apo BCAA dehydrogenase E2 protein] N⁶-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + CO₂ (EC 1.2.1.25; 1.2.4.4)

RR42_RS21785

RR42_RS33045

RR42_RS33050

2-oxoisovalerate dehydrogenase (acylating) (in reverse):
an [apo BCAA dehydrogenase E2 protein] N⁶-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + coenzyme A→an [apo BCAA dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + isobutanoyl-CoA (EC 1.2.1.25; 2.3.1.168)

RR42_RS33055

2-oxoisovalerate dehydrogenase (acylating):
an [apo BCAA dehydrogenase E2 protein] N⁶-dihydrolipoyl-L-lysine + NAD⁺→a [BCAA dehydrogenase E2 protein] N⁶-lipoyl-L-lysine + NADH + H⁺ (EC 1.2.1.25; 1.8.1.4)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information