MetaCyc Pathway: brassinolide biosynthesis II in Cupriavidus basilensis FW507-4G11

Add experiment(s):


campesterol + dioxygen + a reduced [NADPH-hemoprotein reductase]→(22S)-22-hydroxycampesterol + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.178)
No genes
Steroid Delta-isomerase:
campesterol→campest-4-en-3β-ol (EC 5.3.3.1)
RR42_RS31815
(22S)-22-hydroxycampesterol + dioxygen + a reduced [NADPH-hemoprotein reductase]→(22S)-22-hydroxy-campest-4-en-3-one + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.19.79)
No genes
campest-4-en-3β-ol + NAD+→campest-4-en-3-one + NADH + H+ (EC 1.1.1.357)
No genes
(22S)-22-hydroxy-campest-4-en-3-one + dioxygen + a reduced [NADPH-hemoprotein reductase]→(22R,23R)-22,23-dihydroxy-campest-4-en-3-one + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.147)
No genes
Cholestenone 5-alpha-reductase (in reverse):
(22S)-22-hydroxy-campest-4-en-3-one + NADPH + H+→(22S)-22-hydroxy-5α-campestan-3-one + NADP+ (EC 1.3.1.22)
No genes
Cholestenone 5-alpha-reductase (in reverse):
campest-4-en-3-one + NADPH + H+→(5α)-campestan-3-one + NADP+ (EC 1.3.1.22)
No genes
(22S)-22-hydroxy-5α-campestan-3-one + dioxygen + a reduced [NADPH-hemoprotein reductase]→3-dehydro-6-deoxoteasterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.147)
No genes
(22S)-22-hydroxy-5α-campestan-3-one + a reduced two electron carrier→3-epi-6-deoxocathasterone + an oxidized electron carrier (EC 1.1.99.M2)
No genes
Cholestenone 5-alpha-reductase (in reverse):
(22R,23R)-22,23-dihydroxy-campest-4-en-3-one + NADPH + H+→3-dehydro-6-deoxoteasterone + NADP+ (EC 1.3.1.22)
No genes
(5α)-campestan-3-one + a reduced two electron carrier→campestanol + an oxidized electron carrier
No genes
3-epi-6-deoxocathasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→6-deoxotyphasterol + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.147)
No genes
campestanol + dioxygen + a reduced [NADPH-hemoprotein reductase]→6-deoxocathasterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.178)
No genes
6-deoxocathasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→6-deoxoteasterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.147)
No genes
6-deoxoteasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→3-dehydro-6-deoxoteasterone + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.19.79)
No genes
3-dehydro-6-deoxoteasterone + a reduced two electron carrier→6-deoxotyphasterol + an oxidized electron carrier (EC 1.1.99.M2)
No genes
6-deoxotyphasterol + dioxygen + a reduced [NADPH-hemoprotein reductase]→6-deoxocastasterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.M54)
No genes
6-deoxocastasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→6α-hydroxy-6-deoxocastasterone + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.179)
No genes
6α-hydroxy-6-deoxocastasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→castasterone + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.14.179)
No genes
castasterone + dioxygen + a reduced [NADPH-hemoprotein reductase]→brassinolide + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.180)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information