MetaCyc Pathway: NAD de novo biosynthesis II (from tryptophan) in Cupriavidus basilensis FW507-4G11

Tryptophan 2,3-dioxygenase: L-tryptophan + dioxygen→N-formyl-L-kynurenine (EC 1.13.11.11; 1.13.11.52)

RR42_RS15390

Arylformamidase: N-formyl-L-kynurenine + H2O→L-kynurenine + formate + H+ (EC 3.5.1.9)

RR42_RS15380

RR42_RS33590

Kynurenine 3-monooxygenase: L-kynurenine + NADPH + dioxygen + H+→3-hydroxy-L-kynurenine + NADP+ + H2O (EC 1.14.13.9)

No genes

Kynureninase: 3-hydroxy-L-kynurenine + H2O→3-hydroxyanthranilate + L-alanine + H+ (EC 3.7.1.3)

RR42_RS15385

3-hydroxyanthranilate 3,4-dioxygenase: 3-hydroxyanthranilate + dioxygen→2-amino-3-carboxymuconate-6-semialdehyde (EC 1.13.11.6)

RR42_RS05080

2-amino-3-carboxymuconate-6-semialdehyde→quinolinate + H+ + H2O (spontaneous)

RR42_RS05080

Nicotinate-nucleotide diphosphorylase (carboxylating) (in reverse): quinolinate + 2 H+ + 5-phospho-α-D-ribose 1-diphosphate→β-nicotinate D-ribonucleotide + CO2 + diphosphate (EC 2.4.2.19)

RR42_RS17015

RR42_RS21245

Nicotinate-nucleotide adenylyltransferase: β-nicotinate D-ribonucleotide + ATP + H+→nicotinate adenine dinucleotide + diphosphate (EC 2.7.7.18)

RR42_RS04720

NAD(+) synthase (glutamine-hydrolyzing): nicotinate adenine dinucleotide + ATP + L-glutamine + H2O→NAD+ + AMP + L-glutamate + diphosphate + H+ (EC 6.3.5.1)

RR42_RS35170

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information