MetaCyc Pathway: L-leucine biosynthesis in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: L-leucine biosynthesis in Cupriavidus basilensis FW507-4G11

2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H₂O→(2S)-2-isopropylmalate + coenzyme A + H⁺ (EC 2.3.3.13)

3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H₂O (EC 4.2.1.33)

3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H₂O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)

3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD⁺→(2S)-2-isopropyl-3-oxosuccinate + NADH + H⁺ (EC 1.1.1.85)

3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H⁺→4-methyl-2-oxopentanoate + CO₂ (spontaneous) (EC 1.1.1.85)

Branched-chain-amino-acid transaminase (in reverse):
4-methyl-2-oxopentanoate + L-glutamate→L-leucine + 2-oxoglutarate (EC 2.6.1.42; 2.6.1.6)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information