Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9) |
| RR42_RS00885 |
| RR42_RS02515 |
| RR42_RS03010 |
| RR42_RS07610 |
| RR42_RS07645 |
| RR42_RS10260 |
| RR42_RS10540 |
| RR42_RS11085 |
| RR42_RS16595 |
| RR42_RS22625 |
| RR42_RS24300 |
| RR42_RS24970 |
| RR42_RS25210 |
| RR42_RS25380 |
| RR42_RS25455 |
| RR42_RS26090 |
| RR42_RS27160 |
| RR42_RS27235 |
| RR42_RS28055 |
| RR42_RS28390 |
| RR42_RS31890 |
| RR42_RS31920 |
| RR42_RS35915 |
| RR42_RS36320 |
| RR42_RS37020 |
Hydroxymethylglutaryl-CoA synthase:
acetoacetyl-CoA + acetyl-CoA + H2O→(S)-3-hydroxy-3-methylglutaryl-CoA + coenzyme A + H+ (EC 2.3.3.10) |
| No genes |
Hydroxymethylglutaryl-CoA reductase (NADPH) (in reverse):
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+→(R)-mevalonate + coenzyme A + 2 NADP+ (EC 1.1.1.34) |
| No genes |
Mevalonate kinase:
(R)-mevalonate + ATP→(R)-5-phosphomevalonate + ADP + H+ (EC 2.7.1.36) |
| No genes |
Phosphomevalonate kinase:
(R)-5-phosphomevalonate + ATP→(R)-mevalonate diphosphate + ADP (EC 2.7.4.2) |
| No genes |
Diphosphomevalonate decarboxylase:
(R)-mevalonate diphosphate + ATP→3-methylbut-3-en-1-yl diphosphate + ADP + CO2 + phosphate (EC 4.1.1.33) |
| No genes |
Isopentenyl-diphosphate Delta-isomerase:
3-methylbut-3-en-1-yl diphosphate→prenyl diphosphate (EC 5.3.3.2) |
| No genes |
Dimethylallyltranstransferase:
prenyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranyl diphosphate + diphosphate (EC 2.5.1.1) |
| RR42_RS15010 |
Geranyltranstransferase:
geranyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→(2E,6E)-farnesyl diphosphate + diphosphate (EC 2.5.1.10) |
| RR42_RS15010 |
| 2 (2E,6E)-farnesyl diphosphate→presqualene diphosphate + diphosphate (EC 2.5.1.103; 2.5.1.21; 2.5.1.96) |
| RR42_RS07905 |
| RR42_RS26935 |
| RR42_RS32265 |
Squalene synthase:
presqualene diphosphate + NAD(P)H + H+→squalene + NAD(P)+ + diphosphate (EC 2.5.1.21) |
| RR42_RS07905 |
| RR42_RS26935 |
| RR42_RS32265 |
| squalene + dioxygen + a reduced [NADPH-hemoprotein reductase]→(3S)-2,3-epoxy-2,3-dihydrosqualene + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.17) |
| No genes |
Lanosterol synthase:
(3S)-2,3-epoxy-2,3-dihydrosqualene→lanosterol (EC 5.4.99.7) |
| No genes |
| lanosterol + 3 dioxygen + 3 a reduced [NADPH-hemoprotein reductase]→4,4-dimethyl-5-α-cholesta-8,14,24-trien-3-β-ol + formate + 3 an oxidized [NADPH-hemoprotein reductase] + H+ + 4 H2O (EC 1.14.14.154) |
| No genes |
Delta(14)-sterol reductase (in reverse):
4,4-dimethyl-5-α-cholesta-8,14,24-trien-3-β-ol + NADPH + H+→14-demethyllanosterol + NADP+ (EC 1.3.1.70) |
| No genes |
| 14-demethyllanosterol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-4β-methyl-5α-cholesta-8,24-dien-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.9) |
| No genes |
Sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating):
4α-carboxy-4β-methyl-5α-cholesta-8,24-dien-3β-ol + NAD(P)+→3-dehydro-4-methylzymosterol + CO2 + NAD(P)H (EC 1.1.1.170) |
| No genes |
3-keto-steroid reductase (in reverse):
3-dehydro-4-methylzymosterol + NADPH + H+→4α-methyl-zymosterol + NADP+ (EC 1.1.1.270) |
| No genes |
| 4α-methyl-zymosterol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-5α-cholesta-8,24-dien-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.9) |
| No genes |
Sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating):
4α-carboxy-5α-cholesta-8,24-dien-3β-ol + NAD(P)+→zymosterone + CO2 + NAD(P)H (EC 1.1.1.170) |
| No genes |
3-keto-steroid reductase (in reverse):
zymosterone + NADPH + H+→zymosterol + NADP+ (EC 1.1.1.270) |
| No genes |
Sterol 24-C-methyltransferase:
zymosterol + S-adenosyl-L-methionine→fecosterol + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.41) |
| No genes |
Cholestenol Delta-isomerase:
fecosterol→episterol (EC 5.3.3.5) |
| No genes |
| episterol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→ergosta-5,7,24(28)-trien-3β-ol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20) |
| No genes |
| ergosta-5,7,24(28)-trien-3β-ol + dioxygen + a reduced [NADPH-hemoprotein reductase]→ergosta-5,7,22,24(28)-tetraen-3β-ol + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.19.41) |
| No genes |
Delta(24(24(1)))-sterol reductase (in reverse):
ergosta-5,7,22,24(28)-tetraen-3β-ol + NADPH + H+→ergosterol + NADP+ (EC 1.3.1.71) |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information