MetaCyc Pathway: superpathway of branched chain amino acid biosynthesis in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: superpathway of branched chain amino acid biosynthesis in Cupriavidus basilensis FW507-4G11

Acetolactate synthase:
2 pyruvate + H⁺→(S)-2-acetolactate + CO₂ (EC 2.2.1.6)

Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H₂O (EC 4.3.1.19)

Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H⁺→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ (EC 1.1.1.86)

1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H₂O (EC 4.2.1.9)

2-iminobutanoate + H₂O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H₂O→(2S)-2-isopropylmalate + coenzyme A + H⁺ (EC 2.3.3.13)

Acetolactate synthase:
2-oxobutanoate + pyruvate + H⁺→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

Branched-chain-amino-acid transaminase (in reverse):
3-methyl-2-oxobutanoate + L-glutamate→L-valine + 2-oxoglutarate (EC 2.6.1.42)

3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H₂O (EC 4.2.1.33)

Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ (EC 1.1.1.86)

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H₂O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)

3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD⁺→(2S)-2-isopropyl-3-oxosuccinate + NADH + H⁺ (EC 1.1.1.85)

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H⁺→4-methyl-2-oxopentanoate + CO₂ (spontaneous) (EC 1.1.1.85)

Branched-chain-amino-acid transaminase (in reverse):
4-methyl-2-oxopentanoate + L-glutamate→L-leucine + 2-oxoglutarate (EC 2.6.1.42; 2.6.1.6)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information