MetaCyc Pathway: biotin biosynthesis I in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: biotin biosynthesis I in Cupriavidus basilensis FW507-4G11

a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)

No genes

Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H⁺→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H⁺→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H⁺→a glutaryl-[acp] methyl ester + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H⁺→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H⁺→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H⁺→a pimeloyl-[acp] methyl ester + NAD⁺ (EC 1.3.1.9)

a pimeloyl-[acp] methyl ester + H₂O→a pimeloyl-[acp] + methanol + H⁺ (EC 3.1.1.85)

No genes

8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H⁺→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.47)

Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)

Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO₂→dethiobiotin + ADP + 3 H⁺ + phosphate (EC 6.3.3.3)

Biotin synthase:
dethiobiotin + H⁺ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)

Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H⁺ (EC 2.8.1.6)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information