MetaCyc Pathway: 4-hydroxyphenylacetate degradation in Cupriavidus basilensis FW507-4G11

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(4-hydroxyphenyl)acetate + FADH2 + dioxygen→(3,4-dihydroxyphenyl)acetate + FAD + H+ + H2O (EC 1.14.14.9)
No genes
3,4-dihydroxyphenylacetate 2,3-dioxygenase:
(3,4-dihydroxyphenyl)acetate + dioxygen→2-hydroxy-5-carboxymethylmuconate semialdehyde + H+ (EC 1.13.11.15)
RR42_RS27840
5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase:
2-hydroxy-5-carboxymethylmuconate semialdehyde + NAD+ + H2O→5-carboxymethyl-2-hydroxymuconate + NADH + 2 H+ (EC 1.2.1.60)
RR42_RS13660
RR42_RS27835
RR42_RS32650
5-carboxymethyl-2-hydroxymuconate Delta-isomerase:
5-carboxymethyl-2-hydroxymuconate→(3E,5R)-5-carboxy-2-oxohept-3-enedioate (spontaneous) (EC 5.3.3.10)
RR42_RS27845
RR42_RS29125
5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase:
(3E,5R)-5-carboxy-2-oxohept-3-enedioate + H+→(4Z)-2-oxohept-4-enedioate + CO2 (EC 4.1.1.68)
RR42_RS27825
RR42_RS27830
(4Z)-2-oxohept-4-enedioate + H2O→(4S)-4-hydroxy-2-oxoheptanedioate (EC 4.2.1.163)
No genes
(4S)-4-hydroxy-2-oxoheptanedioate→succinate semialdehyde + pyruvate (EC 4.1.2.52)
RR42_RS26640
RR42_RS27275
RR42_RS27855
succinate semialdehyde + NADP+ + H2O→succinate + NADPH + 2 H+ (EC 1.2.1.79)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information