MetaCyc Pathway: reductive TCA cycle II in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Carbon source Deoxyribonucleic from herring sperm 10 mg/mL
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2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
2-oxoglutarate carboxylase:
2-oxoglutarate + ATP + CO2 + H2O→oxalosuccinate + ADP + 2 H+ + phosphate (EC 6.4.1.7)
No genes
oxalosuccinate + NADH + H+→D-threo-isocitrate + NAD+
No genes
Aconitate hydratase (in reverse):
D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
RR42_RS11270 +0.1
RR42_RS14490 -4.1
RR42_RS23515 +0.9
Aconitate hydratase (in reverse):
cis-aconitate + H2O→citrate (EC 4.2.1.3)
RR42_RS11270 +0.1
RR42_RS14490 -4.1
RR42_RS23515 +0.9
Citrate--CoA ligase:
citrate + ATP + coenzyme A→(3S)-citryl-CoA + ADP + phosphate (EC 6.2.1.18)
No genes
Citryl-CoA lyase:
(3S)-citryl-CoA→acetyl-CoA + oxaloacetate (EC 4.1.3.34)
RR42_RS14470 -0.5
RR42_RS24325 -0.2
RR42_RS24350 +0.2
RR42_RS25095 -0.1
RR42_RS26220 -0.7
RR42_RS36610 +0.6
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
RR42_RS02230 -0.5
RR42_RS14465 N.D.
RR42_RS29130 -0.2
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
RR42_RS02195 +0.3
RR42_RS13905 +1.6
RR42_RS27740 +0.1
RR42_RS33090 +2.1
RR42_RS37365 +0.1
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
RR42_RS03095 -1.8
RR42_RS03100 N.D.
RR42_RS31375 -0.1
RR42_RS31380 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information