| Reactions and Genes | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 |
|---|
| remove |
| CO2 + a methanofuran + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→a formylmethanofuran + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.2.7.12) |
| MMP_RS02695 | +0.2 |
| MMP_RS02700 | +0.3 |
| MMP_RS02710 | -0.0 |
| MMP_RS06415 | N.D. |
| MMP_RS06420 | N.D. |
| MMP_RS06425 | N.D. |
| MMP_RS08715 | -5.4 |
Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1) |
| MMP_RS06695 | N.D. |
Formylmethanofuran--tetrahydromethanopterin N-formyltransferase:
a formylmethanofuran + tetrahydromethanopterin + H+→5-formyl-tetrahydromethanopterin + a methanofuran (EC 2.3.1.101) |
| MMP_RS08270 | N.D. |
Methenyltetrahydromethanopterin cyclohydrolase (in reverse):
5-formyl-tetrahydromethanopterin + H+→5,10-methenyltetrahydromethanopterin + H2O (EC 3.5.4.27) |
| MMP_RS06135 | N.D. |
5,10-methenyltetrahydromethanopterin hydrogenase:
5,10-methenyltetrahydromethanopterin + H2→5,10-methylene-tetrahydromethanopterin + H+ (EC 1.12.98.2) |
| MMP_RS00735 | -2.0 |
| 5,10-methylene-tetrahydromethanopterin + a reduced factor 420→5-methyltetrahydromethanopterin + an oxidized factor 420 (EC 1.5.98.2) |
| MMP_RS00340 | N.D. |
| CO2 + 5-methyltetrahydromethanopterin + coenzyme A + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→tetrahydromethanopterin + acetyl-CoA + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 2.3.3.M4) |
| No genes |
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1) |
| MMP_RS07720 | -0.0 |
| MMP_RS07725 | -0.0 |
| MMP_RS07730 | -5.6 |
| MMP_RS07735 | -3.4 |
| MMP_RS07740 | N.D. |
| MMP_RS07745 | N.D. |
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2) |
| MMP_RS05655 | N.D. |
Pyruvate carboxylase:
hydrogen carbonate + pyruvate + ATP→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1) |
| MMP_RS01820 | N.D. |
| MMP_RS01825 | N.D. |
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11) |
| MMP_RS02115 | N.D. |
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31) |
| No genes |
| 2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12) |
| MMP_RS00660 | +0.3 |
| MMP_RS03150 | +0.1 |
| MMP_RS07405 | N.D. |
Glyceraldehyde-3-phosphate dehydrogenase (ferredoxin) (in reverse):
3-phospho-D-glycerate + 3 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→D-glyceraldehyde 3-phosphate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.2.7.6) |
| MMP_RS04900 | -2.4 |
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1) |
| MMP_RS03600 | -5.3 |
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13) |
| MMP_RS01575 | -2.9 |
| MMP_RS01695 | N.D. |
| MMP_RS03595 | N.D. |
ADP-specific phosphofructokinase (in reverse):
β-D-fructofuranose 1,6-bisphosphate + AMP + H+→β-D-fructofuranose 6-phosphate + ADP (EC 2.7.1.146) |
| MMP_RS06675 | -1.5 |
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9) |
| MMP_RS06670 | -5.1 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information