MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes L-Valine (N)
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
RR42_RS05465 -3.0
RR42_RS21205 -0.1
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
RR42_RS01530 -0.1
RR42_RS01535 +0.1
RR42_RS14390 -3.8
RR42_RS14400 -6.0
RR42_RS21080 +0.1
RR42_RS21085 +0.1
RR42_RS22770 +0.0
RR42_RS22775 +0.1
RR42_RS24175 +0.2
RR42_RS24180 -0.0
RR42_RS33965 -0.1
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
RR42_RS01530 -0.1
RR42_RS01535 +0.1
RR42_RS14390 -3.8
RR42_RS14400 -6.0
RR42_RS21080 +0.1
RR42_RS21085 +0.1
RR42_RS22770 +0.0
RR42_RS22775 +0.1
RR42_RS24175 +0.2
RR42_RS24180 -0.0
RR42_RS33965 -0.1
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
RR42_RS14385 -3.4
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
RR42_RS14385 -3.4
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
RR42_RS09745 -0.4
RR42_RS10210 -0.0
RR42_RS10545 -0.7
RR42_RS11005 +0.1
RR42_RS18350 -0.4
RR42_RS24070 -0.1
RR42_RS24560 -0.1
RR42_RS25355 +0.1
RR42_RS25775 N.D.
RR42_RS28650 N.D.
RR42_RS32920 +0.4
RR42_RS34260 +0.1
RR42_RS34620 +0.2
RR42_RS36940 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information