MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Ethanol (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
RR42_RS02175 -0.4
RR42_RS05435 N.D.
RR42_RS05440 N.D.
RR42_RS13670 +0.0
RR42_RS26245 +0.0
RR42_RS29140 +0.1
RR42_RS31370 -0.1
RR42_RS36655 -0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
RR42_RS05445 N.D.
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
RR42_RS16210 N.D.
RR42_RS18585 +0.0
RR42_RS31070 -0.1
RR42_RS33305 -0.4
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
RR42_RS09745 -0.4
RR42_RS10210 -0.1
RR42_RS10545 +0.1
RR42_RS11005 +0.1
RR42_RS18350 -0.2
RR42_RS24070 +0.1
RR42_RS24560 -0.3
RR42_RS25355 -0.0
RR42_RS25775 N.D.
RR42_RS28650 N.D.
RR42_RS32920 +0.0
RR42_RS34260 -1.7
RR42_RS34620 +0.0
RR42_RS36940 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information