MetaCyc Pathway: TCA cycle I (prokaryotic) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes R2A with Cobalt chloride 0.5 mM
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2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
RR42_RS07380 +1.7
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
RR42_RS03095 -0.9
RR42_RS03100 N.D.
RR42_RS31375 -0.2
RR42_RS31380 +0.0
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
RR42_RS14445 N.D.
RR42_RS14455 N.D.
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
RR42_RS02195 -0.3
RR42_RS13905 -2.8
RR42_RS27740 +0.1
RR42_RS33090 -0.0
RR42_RS37365 -0.1
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
RR42_RS02230 +0.2
RR42_RS14465 N.D.
RR42_RS29130 -0.2
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
No genes
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
RR42_RS09325 -0.0
RR42_RS10505 -0.1
RR42_RS14430 N.D.
RR42_RS32385 +0.3
RR42_RS36245 -0.5
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
RR42_RS11270 +0.2
RR42_RS14490 -1.2
RR42_RS23515 +1.9
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
RR42_RS11270 +0.2
RR42_RS14490 -1.2
RR42_RS23515 +1.9
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
RR42_RS17135 +0.0
RR42_RS22955 -0.3
RR42_RS28800 -0.2
RR42_RS29535 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information