MetaCyc Pathway: superpathway of aromatic amino acid biosynthesis in Cupriavidus basilensis FW507-4G11

Reactions and Genes	R2A with Nickel (II) chloride 0.35 mM
3-deoxy-7-phosphoheptulonate synthase: D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
RR42_RS05860	+4.1
3-dehydroquinate synthase: 3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
RR42_RS19005	+2.4
3-dehydroquinate dehydratase: 3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
RR42_RS06175	N.D.
RR42_RS17690	-2.6
RR42_RS32450	+0.1
RR42_RS33730	-0.0
Shikimate dehydrogenase (in reverse): 3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
RR42_RS09460	+0.1
RR42_RS17645	+0.4
RR42_RS21755	+0.6
RR42_RS32445	+0.8
Shikimate kinase: shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
RR42_RS07515	-0.1
RR42_RS19010	+0.5
RR42_RS32495	-0.9
RR42_RS35135	-0.4
3-phosphoshikimate 1-carboxyvinyltransferase: shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
RR42_RS04475	+1.4
Chorismate synthase: 5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
RR42_RS06485	+0.8
Anthranilate synthase: chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
RR42_RS18295	+0.1
RR42_RS18300	-0.1
Chorismate mutase: chorismate→prephenate (EC 5.4.99.5)
RR42_RS04460	+0.3
Prephenate dehydratase: prephenate + H+→3-phenyl-2-oxopropanoate + CO2 + H2O (EC 4.2.1.51)
RR42_RS04460	+0.3
Prephenate dehydrogenase: prephenate + NAD+→3-(4-hydroxyphenyl)pyruvate + CO2 + NADH (EC 1.3.1.12)
RR42_RS04470	+0.3
Anthranilate phosphoribosyltransferase (in reverse): anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
RR42_RS01940	-0.1
RR42_RS18305	-0.0
Phosphoribosylanthranilate isomerase: N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
RR42_RS14365	+0.5
Aspartate transaminase (in reverse): 3-phenyl-2-oxopropanoate + L-glutamate→L-phenylalanine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.57)
RR42_RS01570	-0.0
RR42_RS01590	-0.2
RR42_RS02275	+0.0
RR42_RS04465	+0.2
RR42_RS04985	-0.3
RR42_RS05770	-0.0
RR42_RS05990	+0.1
RR42_RS09190	-0.1
RR42_RS09965	-0.9
RR42_RS10785	+0.2
RR42_RS12545	+0.4
RR42_RS14560	+0.1
RR42_RS15580	+0.1
RR42_RS16470	-0.2
RR42_RS18910	N.D.
RR42_RS21340	+0.1
RR42_RS25980	+0.3
RR42_RS26005	-0.3
RR42_RS26015	-0.2
RR42_RS26185	+0.1
RR42_RS26625	+0.3
RR42_RS28185	+0.1
RR42_RS32165	+0.2
RR42_RS33140	+0.1
RR42_RS33490	+0.2
RR42_RS33805	+0.1
RR42_RS35305	+0.4
RR42_RS36780	+0.2
Aspartate transaminase (in reverse): 3-(4-hydroxyphenyl)pyruvate + L-glutamate→L-tyrosine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
RR42_RS01570	-0.0
RR42_RS01590	-0.2
RR42_RS02275	+0.0
RR42_RS04465	+0.2
RR42_RS04985	-0.3
RR42_RS05770	-0.0
RR42_RS05990	+0.1
RR42_RS09190	-0.1
RR42_RS09965	-0.9
RR42_RS10785	+0.2
RR42_RS12545	+0.4
RR42_RS14560	+0.1
RR42_RS15580	+0.1
RR42_RS16470	-0.2
RR42_RS18910	N.D.
RR42_RS21340	+0.1
RR42_RS25980	+0.3
RR42_RS26005	-0.3
RR42_RS26015	-0.2
RR42_RS26185	+0.1
RR42_RS26625	+0.3
RR42_RS28185	+0.1
RR42_RS32165	+0.2
RR42_RS33140	+0.1
RR42_RS33490	+0.2
RR42_RS33805	+0.1
RR42_RS35305	+0.4
RR42_RS36780	+0.2
Indole-3-glycerol-phosphate synthase: 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
RR42_RS18310	-0.4
Indole-3-glycerol-phosphate lyase: (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
RR42_RS14350	N.D.
RR42_RS14360	+0.7
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
RR42_RS14350	N.D.
RR42_RS14360	+0.7

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information