MetaCyc Pathway: superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis in Methanococcus maripaludis S2

Reactions and Genes	McCas_formate_complete sodium nitrite 0.25 mM
Carbamoyl-phosphate synthase (glutamine-hydrolyzing): L-glutamine + hydrogen carbonate + 2 ATP + H2O→carbamoyl phosphate + 2 ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.5.5)
MMP_RS05240	N.D.
MMP_RS08170	N.D.
Aspartate carbamoyltransferase: carbamoyl phosphate + L-aspartate→N-carbamoyl-L-aspartate + H+ + phosphate (EC 2.1.3.2)
MMP_RS05705	N.D.
MMP_RS08545	N.D.
Dihydroorotase (in reverse): N-carbamoyl-L-aspartate + H+→(S)-dihydroorotate + H2O (EC 3.5.2.3)
MMP_RS05220	N.D.
Dihydroorotate dehydrogenase: (S)-dihydroorotate[in] + an electron-transfer quinone→orotate[in] + an electron-transfer quinol (EC 1.3.5.2)
No genes
Orotate phosphoribosyltransferase (in reverse): orotate + 5-phospho-α-D-ribose 1-diphosphate→orotidine 5'-phosphate + diphosphate (EC 2.4.2.10)
MMP_RS00455	-0.7
MMP_RS07670	N.D.
Orotidine-5'-phosphate decarboxylase: orotidine 5'-phosphate + H+→UMP + CO2 (EC 4.1.1.23)
MMP_RS03170	N.D.
Cytidylate kinase: UMP + ATP→UDP + ADP (EC 2.7.4.14; 2.7.4.22)
MMP_RS02060	N.D.
MMP_RS03290	N.D.
Nucleoside-diphosphate kinase: UDP + ATP→UTP + ADP (EC 2.7.4.6)
MMP_RS01525	-0.1
Ribonucleoside-diphosphate reductase (in reverse): UDP + a reduced thioredoxin→dUDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
No genes
CTP synthase: UTP + L-glutamine + ATP + H2O→CTP + ADP + L-glutamate + 2 H+ + phosphate (EC 6.3.4.2)
MMP_RS04640	N.D.
Nucleoside-diphosphate kinase: dUDP + ATP→dUTP + ADP (EC 2.7.4.6)
MMP_RS01525	-0.1
dUTP diphosphatase: dUTP + H2O→dUMP + diphosphate + H+ (EC 3.6.1.23; 3.6.1.9)
MMP_RS05560	+0.1
Nucleoside-triphosphatase: CTP + H2O→CDP + H+ + phosphate (EC 3.6.1.15; 3.6.1.5)
MMP_RS01180	-0.2
Ribonucleoside-diphosphate reductase (in reverse): CDP + a reduced thioredoxin→dCDP + an oxidized thioredoxin + H2O (EC 1.17.4.1)
No genes
Thymidylate synthase: dUMP + a 5,10-methylenetetrahydrofolate→dTMP + a 7,8-dihydrofolate (EC 2.1.1.45)
MMP_RS05105	-1.8
MMP_RS07105	N.D.
Nucleoside-diphosphate kinase: dCDP + ATP→dCTP + ADP (EC 2.7.4.6)
MMP_RS01525	-0.1
T(2)-induced deoxynucleotide kinase: dTMP + ATP→dTDP + ADP (EC 2.7.4.12; 2.7.4.13; 2.7.4.9)
MMP_RS05350	N.D.
Nucleoside-diphosphate kinase: dTDP + ATP→dTTP + ADP (EC 2.7.4.6)
MMP_RS01525	-0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information