MetaCyc Pathway: methyl tert-butyl ether degradation in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Reactions and Genes Carbon source Phenylacetic 2.5 mM
remove
methyl tert-butyl ether + NADH + dioxygen + H+→tert-butoxymethanol + NAD+ + H2O (EC 1.14.13.M36)
RR42_RS09290 +0.2
RR42_RS20735 N.D.
tert-butoxymethanol + NAD+→tert-butyl formate + NADH + H+ (EC 1.1.1.M26)
No genes
tert-butyl formate + H2O→tert-butyl alcohol + formate + H+
No genes
tert-butyl alcohol + NADPH + dioxygen + H+→2-methylpropane-1,2-diol + NADP+ + H2O (EC 1.14.13.229)
No genes
2-methylpropane-1,2-diol + NAD+→2-hydroxy-2-methylpropanal + NADH + H+ (EC 1.1.1.400)
No genes
2-hydroxy-2-methylpropanal + NAD+ + H2O→2-hydroxy-2-methylpropanoate + NADH + 2 H+ (EC 1.2.1.98)
No genes
2-hydroxy-2-methylpropanoate + ATP + coenzyme A→2-hydroxy-2-methylpropanoyl-CoA + AMP + diphosphate (EC 6.2.1.M4)
No genes
2-hydroxy-2-methylpropanoyl-CoA→(S)-3-hydroxybutanoyl-CoA (EC 5.4.99.64)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
RR42_RS01610 -0.1
RR42_RS02510 +0.0
RR42_RS03005 -0.4
RR42_RS03440 -0.2
RR42_RS05760 -2.6
RR42_RS10265 -0.2
RR42_RS11095 -0.4
RR42_RS22630 +0.0
RR42_RS24305 -0.2
RR42_RS25375 +0.2
RR42_RS28325 +0.1
RR42_RS29465 +0.1
RR42_RS31050 -0.5
RR42_RS35270 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
RR42_RS00885 -0.4
RR42_RS02515 N.D.
RR42_RS03010 -0.1
RR42_RS07610 -0.5
RR42_RS07645 N.D.
RR42_RS10260 -0.0
RR42_RS10540 -0.5
RR42_RS11085 +0.3
RR42_RS16595 +0.1
RR42_RS22625 -0.2
RR42_RS24300 +0.1
RR42_RS24970 +0.1
RR42_RS25210 +0.0
RR42_RS25380 +0.2
RR42_RS25455 +0.1
RR42_RS26090 +0.1
RR42_RS27160 +0.3
RR42_RS27235 -0.1
RR42_RS28055 +0.2
RR42_RS28390 -0.1
RR42_RS31890 -0.3
RR42_RS31920 -0.9
RR42_RS35915 -2.0
RR42_RS36320 -0.5
RR42_RS37020 -0.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information