| Reactions and Genes | Carbon source Phenylacetic 2.5 mM |
|---|
| remove |
Cholesterol oxidase:
cholesterol + dioxygen→cholest-5-en-3-one + hydrogen peroxide (EC 1.1.3.6) |
| RR42_RS25500 | +0.2 |
Steroid Delta-isomerase:
cholest-5-en-3-one→cholest-4-en-3-one (EC 5.3.3.1) |
| RR42_RS31815 | -0.3 |
| cholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-hydroxycholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| (25S)-26-hydroxycholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-oxocholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.29) |
| No genes |
| (25S)-26-oxocholest-4-en-3-one + dioxygen + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-3-oxocholest-4-en-26-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| (25S)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25S)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
| (25S)-3-oxocholest-4-en-26-oyl-CoA + an oxidized electron carrier→3-oxocholest-4,24-dien-26-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxocholest-4,24-dien-26-oyl-CoA + H2O→24-hydroxy-3-oxocholest-4-en-26-oyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
24-hydroxy-3-oxocholest-4-en-26-oyl-CoA + NAD+→3,24-dioxocholest-4-en-26-oyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 | +0.0 |
| RR42_RS03005 | -0.4 |
| RR42_RS03440 | -0.2 |
| RR42_RS05760 | -2.6 |
| RR42_RS10265 | -0.2 |
| RR42_RS11095 | -0.4 |
| RR42_RS22630 | +0.0 |
| RR42_RS25375 | +0.2 |
| RR42_RS28325 | +0.1 |
| RR42_RS29465 | +0.1 |
| RR42_RS31050 | -0.5 |
| RR42_RS35270 | +0.0 |
Propanoyl-CoA C-acyltransferase (in reverse):
3,24-dioxocholest-4-en-26-oyl-CoA + coenzyme A→3-oxochol-4-en-24-oyl-CoA + propanoyl-CoA (EC 2.3.1.176) |
| No genes |
| 3-oxochol-4-en-24-oyl-CoA + an oxidized electron carrier→3-oxochol-4,22-dien-24-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxochol-4,22-dien-24-oyl-CoA + H2O→22-hydroxy-3-oxochol-4-en-24-oyl-CoA |
| No genes |
| 22-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+→3,22-dioxochol-4-en-24-oyl-CoA + NADH + H+ |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
3,22-dioxochol-4-en-24-oyl-CoA + coenzyme A→acetyl-CoA + 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 | -0.4 |
| RR42_RS02515 | N.D. |
| RR42_RS03010 | -0.1 |
| RR42_RS07610 | -0.5 |
| RR42_RS07645 | N.D. |
| RR42_RS10260 | -0.0 |
| RR42_RS10540 | -0.5 |
| RR42_RS11085 | +0.3 |
| RR42_RS16595 | +0.1 |
| RR42_RS22625 | -0.2 |
| RR42_RS24300 | +0.1 |
| RR42_RS24970 | +0.1 |
| RR42_RS25210 | +0.0 |
| RR42_RS25380 | +0.2 |
| RR42_RS25455 | +0.1 |
| RR42_RS26090 | +0.1 |
| RR42_RS27160 | +0.3 |
| RR42_RS28055 | +0.2 |
| RR42_RS31890 | -0.3 |
| RR42_RS31920 | -0.9 |
| RR42_RS35915 | -2.0 |
| RR42_RS36320 | -0.5 |
| RR42_RS37020 | -0.1 |
| 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + an oxidized electron carrier→3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + H2O→17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA |
| No genes |
| 17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA→propanoyl-CoA + androstenedione |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information