| Reactions and Genes | Carbon source Phenylacetic 2.5 mM |
|---|
| remove |
3(or 17)-alpha-hydroxysteroid dehydrogenase:
androsterone + NAD(P)+→5α-androstanedione + NAD(P)H + H+ (EC 1.1.1.209; 1.1.1.213; 1.1.1.357; 1.1.1.50) |
| No genes |
3(or 17)-beta-hydroxysteroid dehydrogenase:
testosterone + NAD(P)+→androstenedione + NAD(P)H + H+ (EC 1.1.1.51) |
| RR42_RS31985 | +0.2 |
3-oxo-5-alpha-steroid 4-dehydrogenase:
5α-androstanedione + an oxidized electron carrier→androstenedione + a reduced two electron carrier (EC 1.3.99.5) |
| No genes |
3-oxosteroid 1-dehydrogenase:
androstenedione + an oxidized electron carrier→androsta-1,4-diene-3,17-dione + a reduced two electron carrier (EC 1.3.99.4) |
| RR42_RS32000 | -0.2 |
| androsta-1,4-diene-3,17-dione + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→9α-hydroxyandrosta-1,4-diene-3,17-dione + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.30) |
| No genes |
| 9α-hydroxyandrosta-1,4-diene-3,17-dione→3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione (spontaneous) |
| No genes |
| 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + dioxygen→3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H+ + H2O (EC 1.14.14.12) |
| No genes |
dioxygenase:
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + dioxygen→(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1 (10),2-dien-4-oate + H+ (EC 1.13.11.25) |
| RR42_RS31960 | +0.3 |
| (1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1 (10),2-dien-4-oate + H2O→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H+ (EC 3.7.1.17) |
| RR42_RS31825 | -0.4 |
| (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxohexanoate (EC 4.2.1.132) |
| RR42_RS31850 | +0.1 |
| 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + ATP + coenzyme A→3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + AMP + diphosphate (EC 6.2.1.41) |
| No genes |
| (S)-4-hydroxy-2-oxohexanoate→1-propanal + pyruvate (EC 4.1.3.43) |
| RR42_RS31860 | -0.1 |
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (in reverse):
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADPH + H+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + NADP+ (EC 1.1.1.145) |
| RR42_RS31900 | +0.4 |
| 1-propanal + coenzyme A + NAD+→propanoyl-CoA + NADH + H+ (EC 1.2.1.87) |
| RR42_RS31855 | +0.1 |
| 3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]propanoyl-CoA + an oxidized electron carrier→(2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + a reduced two electron carrier |
| No genes |
| (2E)-3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]prop-2-enoyl-CoA + H2O→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-hydroxypropanoyl-CoA + NAD+→3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 | +0.0 |
| RR42_RS03005 | -0.4 |
| RR42_RS03440 | -0.2 |
| RR42_RS05760 | -2.6 |
| RR42_RS10265 | -0.2 |
| RR42_RS11095 | -0.4 |
| RR42_RS22630 | +0.0 |
| RR42_RS25375 | +0.2 |
| RR42_RS28325 | +0.1 |
| RR42_RS29465 | +0.1 |
| RR42_RS31050 | -0.5 |
| RR42_RS35270 | +0.0 |
Acetyl-CoA C-acyltransferase (in reverse):
3-[(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-inden-4-yl]-3-oxopropanoyl-CoA + coenzyme A→acetyl-CoA + (3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 | -0.4 |
| RR42_RS02515 | N.D. |
| RR42_RS03010 | -0.1 |
| RR42_RS07610 | -0.5 |
| RR42_RS07645 | N.D. |
| RR42_RS10260 | -0.0 |
| RR42_RS10540 | -0.5 |
| RR42_RS11085 | +0.3 |
| RR42_RS16595 | +0.1 |
| RR42_RS22625 | -0.2 |
| RR42_RS24300 | +0.1 |
| RR42_RS24970 | +0.1 |
| RR42_RS25210 | +0.0 |
| RR42_RS25380 | +0.2 |
| RR42_RS25455 | +0.1 |
| RR42_RS26090 | +0.1 |
| RR42_RS27160 | +0.3 |
| RR42_RS28055 | +0.2 |
| RR42_RS31890 | -0.3 |
| RR42_RS31920 | -0.9 |
| RR42_RS35915 | -2.0 |
| RR42_RS36320 | -0.5 |
| RR42_RS37020 | -0.1 |
| (3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31940 | +0.0 |
| (5R,7aS)-5-hydroxy-7a-methyl-1-oxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NAD+→(5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + NADH + H+ |
| RR42_RS31900 | +0.4 |
| (5R,7aS)-7a-methyl-1,5-dioxo-3,5,6,7-tetrahydro-2H-indene-4-carboxyl-CoA + H2O→(3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H+ |
| RR42_RS31935 | -0.0 |
| (3R)-2-(2-carboxyethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA + H2O→9-carboxy-6-methyl-3,7-dioxononanoyl-CoA |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
9-carboxy-6-methyl-3,7-dioxononanoyl-CoA + coenzyme A→acetyl-CoA + 7-carboxy-4-methyl-5-oxoheptanoyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 | -0.4 |
| RR42_RS02515 | N.D. |
| RR42_RS03010 | -0.1 |
| RR42_RS07610 | -0.5 |
| RR42_RS07645 | N.D. |
| RR42_RS10260 | -0.0 |
| RR42_RS10540 | -0.5 |
| RR42_RS11085 | +0.3 |
| RR42_RS16595 | +0.1 |
| RR42_RS22625 | -0.2 |
| RR42_RS24300 | +0.1 |
| RR42_RS24970 | +0.1 |
| RR42_RS25210 | +0.0 |
| RR42_RS25380 | +0.2 |
| RR42_RS25455 | +0.1 |
| RR42_RS26090 | +0.1 |
| RR42_RS27160 | +0.3 |
| RR42_RS28055 | +0.2 |
| RR42_RS31890 | -0.3 |
| RR42_RS31920 | -0.9 |
| RR42_RS35915 | -2.0 |
| RR42_RS36320 | -0.5 |
| RR42_RS37020 | -0.1 |
| 7-carboxy-4-methyl-5-oxoheptanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + a reduced electron-transfer flavoprotein |
| No genes |
| (2E)-7-carboxy-4-methyl-5-oxohept-2-enoyl-CoA + H2O→(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA (EC 4.2.1.119) |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
(3R)-7-carboxy-3-hydroxy-4-methyl-5-oxoheptanoyl-CoA + NAD+→7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + NADH + H+ (EC 1.1.1.35) |
| RR42_RS02510 | +0.0 |
| RR42_RS03005 | -0.4 |
| RR42_RS03440 | -0.2 |
| RR42_RS05760 | -2.6 |
| RR42_RS10265 | -0.2 |
| RR42_RS11095 | -0.4 |
| RR42_RS22630 | +0.0 |
| RR42_RS25375 | +0.2 |
| RR42_RS28325 | +0.1 |
| RR42_RS29465 | +0.1 |
| RR42_RS31050 | -0.5 |
| RR42_RS35270 | +0.0 |
Acetyl-CoA C-acyltransferase (in reverse):
7-carboxy-4-methyl-3,5-dioxoheptanoyl-CoA + coenzyme A→acetyl-CoA + 5-carboxy-2-methyl-3-oxopentanoyl-CoA (EC 2.3.1.16) |
| RR42_RS00885 | -0.4 |
| RR42_RS02515 | N.D. |
| RR42_RS03010 | -0.1 |
| RR42_RS07610 | -0.5 |
| RR42_RS07645 | N.D. |
| RR42_RS10260 | -0.0 |
| RR42_RS10540 | -0.5 |
| RR42_RS11085 | +0.3 |
| RR42_RS16595 | +0.1 |
| RR42_RS22625 | -0.2 |
| RR42_RS24300 | +0.1 |
| RR42_RS24970 | +0.1 |
| RR42_RS25210 | +0.0 |
| RR42_RS25380 | +0.2 |
| RR42_RS25455 | +0.1 |
| RR42_RS26090 | +0.1 |
| RR42_RS27160 | +0.3 |
| RR42_RS28055 | +0.2 |
| RR42_RS31890 | -0.3 |
| RR42_RS31920 | -0.9 |
| RR42_RS35915 | -2.0 |
| RR42_RS36320 | -0.5 |
| RR42_RS37020 | -0.1 |
| 5-carboxy-2-methyl-3-oxopentanoyl-CoA + coenzyme A→propanoyl-CoA + succinyl-CoA |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information