MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Carbon source Phenylacetic 2.5 mM
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Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
RR42_RS02570 +0.2
RR42_RS07270 -0.0
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
RR42_RS27010 -0.1
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
RR42_RS31950 -0.8
RR42_RS31955 +0.2
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
RR42_RS00730 +0.2
RR42_RS02510 +0.0
RR42_RS02525 -0.1
RR42_RS03015 -0.1
RR42_RS04550 -0.2
RR42_RS05755 -0.1
RR42_RS10250 -0.5
RR42_RS10265 -0.2
RR42_RS10270 +0.2
RR42_RS11095 -0.4
RR42_RS12500 -0.1
RR42_RS12795 +0.4
RR42_RS13545 N.D.
RR42_RS16175 +0.5
RR42_RS16790 -0.2
RR42_RS18250 -1.5
RR42_RS19805 -0.2
RR42_RS22630 +0.0
RR42_RS22680 -0.8
RR42_RS23295 +0.4
RR42_RS23450 +0.1
RR42_RS23710 -0.3
RR42_RS24355 -0.1
RR42_RS24895 +0.0
RR42_RS25480 +0.4
RR42_RS25525 +0.0
RR42_RS25900 -0.1
RR42_RS28050 N.D.
RR42_RS28325 +0.1
RR42_RS28330 +0.5
RR42_RS29425 -0.3
RR42_RS29465 +0.1
RR42_RS29940 -0.0
RR42_RS29945 -0.0
RR42_RS31580 +0.3
RR42_RS31935 -0.0
RR42_RS31945 -0.1
RR42_RS34175 -0.3
RR42_RS34180 +0.1
RR42_RS34905 -0.1
RR42_RS35270 +0.0
RR42_RS36495 +0.4
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
RR42_RS10720 +0.1
RR42_RS14835 -0.0
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
RR42_RS01610 -0.1
RR42_RS02510 +0.0
RR42_RS03005 -0.4
RR42_RS03440 -0.2
RR42_RS05760 -2.6
RR42_RS10265 -0.2
RR42_RS11095 -0.4
RR42_RS22630 +0.0
RR42_RS24305 -0.2
RR42_RS25375 +0.2
RR42_RS28325 +0.1
RR42_RS29465 +0.1
RR42_RS31050 -0.5
RR42_RS35270 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
RR42_RS00885 -0.4
RR42_RS02515 N.D.
RR42_RS03010 -0.1
RR42_RS07610 -0.5
RR42_RS07645 N.D.
RR42_RS10260 -0.0
RR42_RS10540 -0.5
RR42_RS11085 +0.3
RR42_RS16595 +0.1
RR42_RS22625 -0.2
RR42_RS24300 +0.1
RR42_RS24970 +0.1
RR42_RS25210 +0.0
RR42_RS25380 +0.2
RR42_RS25455 +0.1
RR42_RS26090 +0.1
RR42_RS27160 +0.3
RR42_RS27235 -0.1
RR42_RS28055 +0.2
RR42_RS28390 -0.1
RR42_RS31890 -0.3
RR42_RS31920 -0.9
RR42_RS35915 -2.0
RR42_RS36320 -0.5
RR42_RS37020 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information