MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Carbon source Phenylacetic 2.5 mM
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
RR42_RS00885 -0.2
RR42_RS02515 N.D.
RR42_RS03010 -0.3
RR42_RS07610 -0.8
RR42_RS07645 N.D.
RR42_RS10260 -0.5
RR42_RS10540 -0.3
RR42_RS11085 +0.3
RR42_RS16595 -0.1
RR42_RS22625 +0.1
RR42_RS24300 +0.0
RR42_RS24970 -0.1
RR42_RS25210 -0.1
RR42_RS25380 -0.0
RR42_RS25455 +0.3
RR42_RS26090 +0.1
RR42_RS27160 +0.0
RR42_RS27235 +0.1
RR42_RS28055 +0.1
RR42_RS28390 -0.0
RR42_RS31890 +0.1
RR42_RS31920 -0.5
RR42_RS35915 -1.8
RR42_RS36320 -0.2
RR42_RS37020 -0.0
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
RR42_RS01610 -0.1
RR42_RS02510 -0.0
RR42_RS03005 -0.6
RR42_RS03440 -0.1
RR42_RS05760 -2.7
RR42_RS10265 -0.0
RR42_RS11095 -0.5
RR42_RS22630 -0.2
RR42_RS24305 +0.1
RR42_RS25375 -0.4
RR42_RS28325 -0.0
RR42_RS29465 +0.1
RR42_RS31050 -0.4
RR42_RS35270 -0.1
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
RR42_RS07615 -0.2
RR42_RS26085 +0.0
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information