MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Carbon source Phenylacetic 2.5 mM
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
RR42_RS00885 -0.2
RR42_RS02515 N.D.
RR42_RS03010 -0.3
RR42_RS07610 -0.8
RR42_RS07645 N.D.
RR42_RS10260 -0.5
RR42_RS10540 -0.3
RR42_RS11085 +0.3
RR42_RS16595 -0.1
RR42_RS22625 +0.1
RR42_RS24300 +0.0
RR42_RS24970 -0.1
RR42_RS25210 -0.1
RR42_RS25380 -0.0
RR42_RS25455 +0.3
RR42_RS26090 +0.1
RR42_RS27160 +0.0
RR42_RS27235 +0.1
RR42_RS28055 +0.1
RR42_RS28390 -0.0
RR42_RS31890 +0.1
RR42_RS31920 -0.5
RR42_RS35915 -1.8
RR42_RS36320 -0.2
RR42_RS37020 -0.0
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
RR42_RS01610 -0.1
RR42_RS02510 -0.0
RR42_RS03005 -0.6
RR42_RS03440 -0.1
RR42_RS05760 -2.7
RR42_RS10265 -0.0
RR42_RS11095 -0.5
RR42_RS22630 -0.2
RR42_RS24305 +0.1
RR42_RS25375 -0.4
RR42_RS28325 -0.0
RR42_RS29465 +0.1
RR42_RS31050 -0.4
RR42_RS35270 -0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
RR42_RS00730 +0.2
RR42_RS02510 -0.0
RR42_RS02525 -0.3
RR42_RS03015 -0.2
RR42_RS04550 +0.1
RR42_RS05755 -0.7
RR42_RS10250 +0.2
RR42_RS10265 -0.0
RR42_RS10270 -0.1
RR42_RS11095 -0.5
RR42_RS12500 +0.4
RR42_RS12795 -0.0
RR42_RS13545 N.D.
RR42_RS16175 +0.4
RR42_RS16790 -0.1
RR42_RS18250 -1.7
RR42_RS19805 -0.0
RR42_RS22630 -0.2
RR42_RS22680 -0.4
RR42_RS23295 +0.3
RR42_RS23450 +0.3
RR42_RS23710 -0.2
RR42_RS24355 -0.3
RR42_RS24895 -0.5
RR42_RS25480 -0.2
RR42_RS25525 -0.0
RR42_RS25900 +0.1
RR42_RS28050 N.D.
RR42_RS28325 -0.0
RR42_RS28330 +0.1
RR42_RS29425 -0.2
RR42_RS29465 +0.1
RR42_RS29940 +0.1
RR42_RS29945 -0.2
RR42_RS31580 +0.0
RR42_RS31935 +0.3
RR42_RS31945 +0.2
RR42_RS34175 +0.1
RR42_RS34180 -0.0
RR42_RS34905 -0.1
RR42_RS35270 -0.1
RR42_RS36495 +0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
RR42_RS11345 -0.1
RR42_RS13540 -0.2
RR42_RS16575 -0.3
RR42_RS16795 +0.1
RR42_RS20905 N.D.
RR42_RS21420 -1.2
RR42_RS23155 -0.1
RR42_RS23700 -0.4
RR42_RS24795 -0.1
RR42_RS24965 -0.1
RR42_RS25105 +0.2
RR42_RS25910 -0.0
RR42_RS25970 +0.2
RR42_RS28565 -0.3
RR42_RS28610 +0.1
RR42_RS29500 -0.2
RR42_RS31105 +0.0
RR42_RS31905 -0.3
RR42_RS31910 -2.4
RR42_RS31925 N.D.
RR42_RS31930 -0.3
RR42_RS36460 +0.3
RR42_RS36490 +0.1
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information