MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Cupriavidus basilensis FW507-4G11

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Reactions and Genes adipate (C)
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
RR42_RS26685 -0.7
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
RR42_RS05495 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
RR42_RS03215 N.D.
RR42_RS16500 +0.7
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
RR42_RS03215 N.D.
RR42_RS16500 +0.7
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
RR42_RS05195 N.D.
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
RR42_RS17575 N.D.
RR42_RS33720 -0.2
RR42_RS33725 -0.0
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
RR42_RS17575 N.D.
RR42_RS33720 -0.2
RR42_RS33725 -0.0
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
RR42_RS13045 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
RR42_RS18285 N.D.
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
No genes
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
No genes
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
RR42_RS01825 N.D.
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
RR42_RS03200 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
RR42_RS06175 N.D.
RR42_RS33730 +0.0
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
RR42_RS03210 +0.2
RR42_RS19870 +0.1
RR42_RS33735 +0.1
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
RR42_RS07365 +0.1
RR42_RS07370 N.D.
RR42_RS07380 -1.3
RR42_RS10660 +0.1
RR42_RS21775 -0.0
RR42_RS24415 -0.0
RR42_RS26120 +0.1
RR42_RS26125 -0.1
RR42_RS32980 -0.1
RR42_RS33045 +0.3
RR42_RS34220 -0.1
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
RR42_RS00885 +0.1
RR42_RS02515 N.D.
RR42_RS03010 -0.3
RR42_RS07610 +1.9
RR42_RS07645 N.D.
RR42_RS10260 -0.4
RR42_RS10540 -0.2
RR42_RS11085 +0.1
RR42_RS16595 +0.0
RR42_RS22625 -0.2
RR42_RS24300 -0.1
RR42_RS24970 +0.1
RR42_RS25210 -0.2
RR42_RS25380 -0.2
RR42_RS25455 +0.2
RR42_RS26090 -0.1
RR42_RS27160 +0.1
RR42_RS27235 +0.1
RR42_RS28055 -0.2
RR42_RS28390 +0.0
RR42_RS31890 -0.3
RR42_RS31920 -1.0
RR42_RS35915 -1.3
RR42_RS36320 +0.1
RR42_RS37020 +0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
RR42_RS01610 +0.3
RR42_RS02510 +0.4
RR42_RS03005 -0.0
RR42_RS03440 +0.3
RR42_RS05760 +0.2
RR42_RS10265 +0.0
RR42_RS11095 +2.8
RR42_RS22630 -0.6
RR42_RS24305 +0.3
RR42_RS25375 +0.4
RR42_RS28325 -0.0
RR42_RS29465 +0.1
RR42_RS31050 -0.1
RR42_RS35270 +0.3
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
RR42_RS00730 -0.0
RR42_RS02510 +0.4
RR42_RS02525 -0.0
RR42_RS03015 +0.0
RR42_RS04550 +0.0
RR42_RS05755 -0.2
RR42_RS10250 +0.1
RR42_RS10265 +0.0
RR42_RS10270 -0.1
RR42_RS11095 +2.8
RR42_RS12500 +0.1
RR42_RS12795 -0.3
RR42_RS13545 N.D.
RR42_RS16175 +0.1
RR42_RS16790 -0.3
RR42_RS18250 -0.0
RR42_RS19805 -0.0
RR42_RS22630 -0.6
RR42_RS22680 +0.3
RR42_RS23295 -0.1
RR42_RS23450 -0.3
RR42_RS23710 +0.1
RR42_RS24355 +1.8
RR42_RS24895 +0.1
RR42_RS25480 -0.6
RR42_RS25525 -0.2
RR42_RS25900 -0.2
RR42_RS28050 N.D.
RR42_RS28325 -0.0
RR42_RS28330 -0.5
RR42_RS29425 -0.1
RR42_RS29465 +0.1
RR42_RS29940 +0.2
RR42_RS29945 +0.3
RR42_RS31580 +0.1
RR42_RS31935 -0.0
RR42_RS31945 -0.9
RR42_RS34175 +0.9
RR42_RS34180 +0.5
RR42_RS34905 -0.1
RR42_RS35270 +0.3
RR42_RS36495 +0.6
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
RR42_RS11345 -0.4
RR42_RS13540 +0.9
RR42_RS16575 +0.1
RR42_RS16795 +0.0
RR42_RS20905 N.D.
RR42_RS21420 -3.0
RR42_RS23155 -0.2
RR42_RS23700 -0.2
RR42_RS24795 +0.0
RR42_RS24965 -0.2
RR42_RS25105 +0.4
RR42_RS25910 -0.1
RR42_RS25970 +0.6
RR42_RS28565 -0.4
RR42_RS28610 -0.4
RR42_RS29500 -0.2
RR42_RS31105 +0.0
RR42_RS31905 -0.3
RR42_RS31910 -0.5
RR42_RS31925 N.D.
RR42_RS31930 -0.0
RR42_RS36460 +0.3
RR42_RS36490 +0.1
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information